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Anti-BCL2 Antibody (4Z465) (Synonyms: PPP1R50, Bcl-2, B-cell CLL/lymphoma 2)

Catalog No. TMAC-00394 Copy Product Info
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Anti-BCL2 Antibody (4Z465) is a Rabbit antibody targeting BCL2. Anti-BCL2 Antibody (4Z465) can be used in FCM,ICC/IF,IHC,WB.

Anti-BCL2 Antibody (4Z465)

Copy Product Info
🥰Excellent
Catalog No. TMAC-00394
Synonyms PPP1R50, Bcl-2, B-cell CLL/lymphoma 2

Anti-BCL2 Antibody (4Z465) is a Rabbit antibody targeting BCL2. Anti-BCL2 Antibody (4Z465) can be used in FCM,ICC/IF,IHC,WB.

Anti-BCL2 Antibody
(4Z465)
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2977-10 days7-10 days
100 μL$4967-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-BCL2 Antibody (4Z465) is a Rabbit antibody targeting BCL2. Anti-BCL2 Antibody (4Z465) can be used in FCM,ICC/IF,IHC,WB.
SynonymsPPP1R50, Bcl-2, B-cell CLL/lymphoma 2
Ig Type
IgG
Clone
4Z465
Reactivity
Human,Mouse
Verified Activity
1. Western blot analysis of Bcl-2 on different lysates using anti-Bcl-2 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela, Lane 2: Jurkat, Lane 3: MCF-7.
2. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Bcl-2 antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Bcl-2 antibody. Counter stained with hematoxylin.
4. Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Bcl-2 antibody. Counter stained with hematoxylin.
5. Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Bcl-2 antibody. Counter stained with hematoxylin.
6. ICC staining Bcl-2 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
7. ICC staining Bcl-2 in A549 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
8. Flow cytometric analysis of Jurkat cells with Bcl-2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
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Application
Recommended Dose
WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionRecombinant Antibody
PurificationProA affinity purified
AppearanceLiquid
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Research BackgroundBcl-2 is one among many key regulators of apoptosis, which are essential for proper development, tissue homeostasis, and protection against foreign pathogens. Human Bcl-2 is an apoptotic, membrane-associated oncoprotein that can promote cell survival through protein-protein interactions with other Bcl-2 related family members, such as the death suppressors Bcl-xL, Mcl-1, Bcl-w, and A1 or the death agonists Bax, Bak, Bik, Bad, and Bid. The apoptotic function of Bcl-2 can also be regulated through proteolytic processing and phospho-rylation. Bcl-2 may promote cell survival by interfering with the activation of the cytochrome c/Apaf-1 pathway through stabilization of the mitochondrial membrane. Mutations in the Bcl-2 gene can contribute to cancers where normal physiological cell death mechanisms are compromised by deregulation of the apoptotic influence of Bcl-2.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 26/22 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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