Anti-BCL2 Antibody (2P100) is a Mouse antibody targeting BCL2. Anti-BCL2 Antibody (2P100) can be used in IF, IHC-Fr, IHC-P, WB.

IgG

2P100

Human,Rat (predicted:Mouse,Dog,Pig,Cow,Horse,Rabbit,Sheep,GuineaPig)

1. Sample:
Jurkat (Human) Cell Lysate at 30 μg
Hela (Human) Cell Lysate at 30 μg
HL60 (Human) Cell Lysate at 30 μg
Primary: Anti-Bcl-2 (TMAB-00194) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 26 kDa
Observed band size: 26 kDa
2. Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Bcl-2) Polyclonal Antibody, Unconjugated (TMAB-00194) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Bcl-2) Monoclonal Antibody, Unconjugated (TMAB-00194) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Bcl-2) Monoclonal Antibody, Unconjugated (TMAB-00194) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Bcl-2) Monoclonal Antibody, Unconjugated (TMAB-00194) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
6. Sample:
LOVO Cell (Human) Lysate at 40 μg
MCF-7 Cell (Human) Lysate at 40 μg
Primary: Anti-Bcl-2 (TMAB-00194) at 1/2 000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 26 kDa
Observed band size: 28 kDa
7. Sample:
LOVO (Human) Cell Lysate at 30 μg
Primary: Anti-Bcl-2 (TMAB-00194) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 26 kDa
Observed band size: 26 kDa

IF=1:100-500; IHC-Fr=1:100-500; IHC-P=1:100-500; WB=1:500-2000

Monoclonal

KLH conjugated synthetic peptide: human Bcl-2

Human

BCL2

Apoptosis regulator Bcl-2

Cellular metabolic process,Response to hypoxia,Bcl 2 family,Metabolism,Bcl 2 family,Cell survival & death,Oncoproteins,Bcl2 Family,Apoptosis marker and proteins ELISA kits,Apoptosis,Hypoxia,Mitochondrial markers,Cancer,Mitochondrial

Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).