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Anti-BCL-XL Antibody (4G501)

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Catalog No. TMAY-03026
Alias Bcl-XL, bcl-x, BclX, BCL2-like 1, bcl2-L-1, Bcl2l, Bcl(X)L

Anti-BCL-XL Antibody (4G501) is a Rabbit antibody targeting BCL-XL. Anti-BCL-XL Antibody (4G501) can be used in WB,IP.

Anti-BCL-XL Antibody (4G501)

Anti-BCL-XL Antibody (4G501)

😃Good
Catalog No. TMAY-03026Alias Bcl-XL, bcl-x, BclX, BCL2-like 1, bcl2-L-1, Bcl2l, Bcl(X)L
Anti-BCL-XL Antibody (4G501) is a Rabbit antibody targeting BCL-XL. Anti-BCL-XL Antibody (4G501) can be used in WB,IP.
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100 μL$269 7-10 days
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Product Introduction

Bioactivity
Description
Anti-BCL-XL Antibody (4G501) is a Rabbit antibody targeting BCL-XL. Anti-BCL-XL Antibody (4G501) can be used in WB,IP.
AliasBcl-XL, bcl-x, BclX, BCL2-like 1, bcl2-L-1, Bcl2l, Bcl(X)L
Ig Type
Monoclonal Rabbit IgG
Clone
4G501
Reactivity
Mouse
Verified Activity
1. Mouse BCL2L1 was immunoprecipitated using:

-Lane A:0.5 mg A549 Whole Cell Lysate.

-Lane B:0.5 mg K562 Whole Cell Lysate.

-2 µL anti-Mouse BCL2L1 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

-Primary antibody:

-Anti-Mouse BCL2L1 rabbit monoclonal antibody, at 1:200 dilution.

-Secondary antibody:

-Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution.

-Developed using the odyssey technique.

-Performed under reducing conditions.

-Predicted band size: 26 kDa.

-Observed band size: 30 kDa.

2. Anti-BCL2L1 rabbit monoclonal antibody at 1:500 dilution.

-Lane A: Jurkat Whole Cell Lysate.

-Lane B: MCF7 Whole Cell Lysate.

-Lane C: K562 Whole Cell lysate.

-Lysates/proteins at 30 μg per lane.

-Secondary

-Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

-Developed using the Odyssey technique.

-Performed under reducing conditions.

-Predicted band size:26 kDa.

-Observed band size:30 kDa
Application
WB,IP
Recommended Dose
WB: 1:500-1:2000; IP: 1-4 μL/mg of lysate
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionThis antibody was obtained from a rabbit immunized with purified, recombinant Mouse BCL2L1 / Bcl-XL (rM BCL2L1 / Bcl-XL; TMPY-02564; Q64373-1; Met1-Arg212).
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundB-cell lymphoma-extra large (Bcl-xl) is a transmembrane molecule in the mitochondria. Bcl-xL (BCL2L1), belongs to the Bcl-2 family. Members of the bcl-2 family encode proteins that function either to promote or to inhibit apoptosis. Antiapoptotic members such as Bcl-2 and Bcl-xL prevent PCD in response to a wide variety of stimuli to take part in cancer survival. Conversely, proapoptotic proteins, exemplified by Bax and Bak, can accelerate death and in some instances are sufficient to cause apoptosis independent of additional signals. The crystal and solution structures of a Bcl-2 family member, Bcl-xL is like this: The structures consist of two central, primarily hydrophobic α-helices, which are surrounded by amphipathic helices. A 60-residue loop connecting helices αl and α2 was found to be flexible and non-essential for anti-apoptotic activity. Bcl-xL is characterized as an important factor in autophagy, inhibiting Beclin 1-mediated autophagy by binding to Beclin 1. In addition, Beclin 1, Bcl-2 and Bcl-xL can cooperate with Atg5 or Ca2+to regulate both autophagy and apoptosis. Bcl-xL is also implicated in anoxia induced cell death. The pathway is initiated by the loss of function of the prosurvival Bcl-2 family members Mcl-1 and Bcl-2 / Bcl-XL, resulting in Bax- or Bak-dependent release of cytochrome c and subsequent caspase-9-dependent cell death. Thus, Bcl-xL, the well-characterized apoptosis guards, appears to be important in cell death.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Mouse BCL2L1 / Bcl-XL Protein (TMPY-02564)
Antigen Species
Mouse
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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