Anti-Bcl-2 Polyclonal Antibody is a Rabbit antibody targeting Bcl-2. Anti-Bcl-2 Polyclonal Antibody can be used in WB,ICC/IF.

IgG

Human,Mouse,Rat

1. Sample:
Lane 1: Spleen (Rat) Lysate at 40 μg
Lane 2: Bone (Rat) Lysate at 40 μg
Primary: Anti-Bcl-2 (TMAB-03056) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 26 kD
Observed band size: 26 kD
2. Sample:
Lane 1: Spleen (Mouse) Lysate at 40 μg
Lane 2: Lymph node (Mouse) Lysate at 40 μg
Lane 3: Bone (Mouse) Lysate at 40 μg
Lane 4: Thyroid gland (Mouse) Lysate at 40 μg
Lane 5: Spleen (Rat) Lysate at 40 μg
Primary: Anti-Bcl-2 (TMAB-03056) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 26 kD
Observed band size: 26 kD
3. 4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (Bcl-2) polyclonal Antibody, unconjugated (TMAB-03056) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

WB: 1:1000-2000; ICC/IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human Bcl-2

Human

BCL2

Apoptosis regulator Bcl-2

Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).