Anti-Bcl-2 Antibody (9K951) is a Rabbit antibody targeting Bcl-2. Anti-Bcl-2 Antibody (9K951) can be used in FCM, ICC/IF, IF, IHC-Fr, IHC-P, WB.

IgG

9K951

Human

1. Paraformaldehyde-fixed, paraffin embedded Human tonsils; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Bcl-2 Monoclonal Antibody, Unconjugated (TMAB-03059) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Human lymph; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Bcl-2 Monoclonal Antibody, Unconjugated (TMAB-03059) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Bcl-2 Monoclonal Antibody, Unconjugated (TMAB-03059) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Bcl-2 Monoclonal Antibody, Unconjugated (TMAB-03059) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
5. 25 ug total protein per lane of various lysates (see on figure) probed with Bcl-2 monoclonal antibody, unconjugated (TMAB-03059) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
6. The Hela (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-Bcl-2 antibody (TMAB-03059): 1 μg/10^6 cells; Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.
7. 4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (Bcl-2) monoclonal Antibody, unconjugated (TMAB-03059) 1: 100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, BF488) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

FCM=1 μg/Test; ICC/IF=1:50-200; IF=1:200-500; IHC-Fr=1:200-500; IHC-P=1:200-500; WB=1:1000-5000

Monoclonal

Recombinant Protein: human Bcl-2 protein

Human

BCL2

Apoptosis regulator Bcl-2

Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).