Anti-Bcl-2 Antibody (7L839) is a Mouse antibody targeting Bcl-2. Anti-Bcl-2 Antibody (7L839) can be used in WB,ICC/IF,FCM.

IgG1

7L839

Human

1. 25 μg total protein per lane of various lysates (see on figure) probed with Bcl-2 monoclonal antibody, unconjugated (TMAB-03054) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. The Hela (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.). Primary Antibody (green): Mouse Anti-Bcl-2 antibody (TMAB-03054): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Mouse IgG-BF488: 1 μg/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.
3. 4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (Bcl-2) monoclonal Antibody, unconjugated (TMAB-03054) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Mouse IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

WB: 1:1000-5000; ICC/IF: 1:100-500; FCM: 1μg/Test

Monoclonal

Recombinant Protein: human Bcl-2 protein

Human

BCL2

Apoptosis regulator Bcl-2

Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).