Anti-BBC3/PUMA Polyclonal Antibody is a Rabbit antibody targeting BBC3/PUMA. Anti-BBC3/PUMA Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.

IgG

Human,Rat (predicted:Mouse,Dog,Pig,Cow)

1. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-BBC3/PUMA Polyclonal Antibody, Unconjugated (TMAB-00192) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Sample: K562 Cell Lysate at 30 μg
Primary: Anti-BBC3 (TMAB-00192) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 21 kDa
Observed band size: 25 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human BBC3

Human

BBC3

Bcl-2-binding component 3, isoforms 1/2

Bcl 2 family,Metabolism,Bcl 2 family,Bcl2 Family,Apoptosis,Mitochondrial markers,Cancer,Mitochondrial

Essential mediator of p53/TP53-dependent and p53/TP53-independent apoptosis. Functions by promoting partial unfolding of BCL2L1 and dissociation of BCL2L1 from p53/TP53. Essential for ER stress-induced cell death.