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Anti-BBC3/PUMA Polyclonal Antibody
😃Good
Catalog No. TMAB-00192
Alias PUMA/JFY1, PUMA alpha, PUMA, p53 Upregulated Modulator of Apoptosis, p53 up regulated modulator of apoptosis, JFY1, JFY 1, bcl-2 binding component 3, BCL2 binding component 3, BCL 2 binding component 3, BBC3_RAT, BBC3, BBC 3
Anti-BBC3/PUMA Polyclonal Antibody is a Rabbit antibody targeting BBC3/PUMA. Anti-BBC3/PUMA Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-BBC3/PUMA Polyclonal Antibody
Anti-BBC3/PUMA Polyclonal Antibody
😃Good
Catalog No. TMAB-00192Alias PUMA/JFY1, PUMA alpha, PUMA, p53 Upregulated Modulator of Apoptosis, p53 up regulated modulator of apoptosis, JFY1, JFY 1, bcl-2 binding component 3, BCL2 binding component 3, BCL 2 binding component 3, BBC3_RAT, BBC3, BBC 3
Anti-BBC3/PUMA Polyclonal Antibody is a Rabbit antibody targeting BBC3/PUMA. Anti-BBC3/PUMA Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $374 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-BBC3/PUMA Polyclonal Antibody is a Rabbit antibody targeting BBC3/PUMA. Anti-BBC3/PUMA Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Alias | PUMA/JFY1, PUMA alpha, PUMA, p53 Upregulated Modulator of Apoptosis, p53 up regulated modulator of apoptosis, JFY1, JFY 1, bcl-2 binding component 3, BCL2 binding component 3, BCL 2 binding component 3, BBC3_RAT, BBC3, BBC 3 |
| Ig Type | IgG |
| Reactivity | Human,Rat (predicted:Mouse,Dog,Pig,Cow) |
| Verified Activity | 1. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-BBC3/PUMA Polyclonal Antibody, Unconjugated (TMAB-00192) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 2. Sample: K562 Cell Lysate at 30 μg Primary: Anti-BBC3 (TMAB-00192) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 21 kDa Observed band size: 25 kDa |
| Application | WB,IHC-P,IHC-Fr,IF |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Mitochondrion. Note=Localized to the mitochondria in order to induce cytochrome c release. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Immunogen | KLH conjugated synthetic peptide: human BBC3 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Bcl 2 family,Metabolism,Bcl 2 family,Bcl2 Family,Apoptosis,Mitochondrial markers,Cancer,Mitochondrial |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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