Anti-BBC3/PUMA Polyclonal Antibody is a Rabbit antibody targeting BBC3/PUMA. Anti-BBC3/PUMA Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Rat (predicted:Mouse,Dog,Pig,Cow)

1. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-BBC3/PUMA Polyclonal Antibody, Unconjugated (TMAB-00192) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Sample: K562 Cell Lysate at 30 μg
Primary: Anti-BBC3 (TMAB-00192) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 21 kDa
Observed band size: 25 kDa

WB,IHC-P,IHC-Fr,IF

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human BBC3

Human

Bcl 2 family,Metabolism,Bcl 2 family,Bcl2 Family,Apoptosis,Mitochondrial markers,Cancer,Mitochondrial