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Anti-BBC3/PUMA Polyclonal Antibody

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Catalog No. TMAB-00192

Anti-BBC3/PUMA Polyclonal Antibody is a Rabbit antibody targeting BBC3/PUMA. Anti-BBC3/PUMA Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-BBC3/PUMA Polyclonal Antibody

Anti-BBC3/PUMA Polyclonal Antibody

😃Good
Catalog No. TMAB-00192
Anti-BBC3/PUMA Polyclonal Antibody is a Rabbit antibody targeting BBC3/PUMA. Anti-BBC3/PUMA Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$220 7-10 days
100 μL$374 7-10 days
200 μL$529 7-10 days
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Product Introduction

Bioactivity
Description
Anti-BBC3/PUMA Polyclonal Antibody is a Rabbit antibody targeting BBC3/PUMA. Anti-BBC3/PUMA Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human,Rat (predicted:Mouse,Dog,Pig,Cow)
Verified Activity
1. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-BBC3/PUMA Polyclonal Antibody, Unconjugated (TMAB-00192) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Sample: K562 Cell Lysate at 30 μg
Primary: Anti-BBC3 (TMAB-00192) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 21 kDa
Observed band size: 25 kDa
Application
WB,IHC-P,IHC-Fr,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationMitochondrion. Note=Localized to the mitochondria in order to induce cytochrome c release.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human BBC3
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Bcl 2 family,Metabolism,Bcl 2 family,Bcl2 Family,Apoptosis,Mitochondrial markers,Cancer,Mitochondrial
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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