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Anti-BAMBI Polyclonal Antibody is a Rabbit antibody targeting BAMBI. Anti-BAMBI Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $221 | 7-10 days | |
100 μL | $373 | 7-10 days | |
200 μL | $529 | 7-10 days |
Description | Anti-BAMBI Polyclonal Antibody is a Rabbit antibody targeting BAMBI. Anti-BAMBI Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
Alias | BMP and activin membrane-bound inhibitor, 2610003H06Rik |
Ig Type | IgG |
Reactivity | Human,Mouse (predicted:Rat,Dog,Pig,Cow,Rabbit,Sheep,GuineaPig) |
Verified Activity | 1. Sample: Testis (Mouse) Lysate at 40 μg Spleen (Mouse) Lysate at 40 μg Primary: Anti-BAMBI (TMAB-00184) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 27 kDa Observed band size: 30 kDa 2. Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-BAMBI Polyclonal Antibody, Unconjugated (TMAB-00184) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 3. Sample: Lane 1: K562 (Human) Cell Lysate at 30 μg Lane 2: MCF-7 (Human) Cell Lysate at 30 μg Lane 3: HepG2 (Human) Cell Lysate at 30 μg Lane 4: A549 (Human) Cell Lysate at 30 μg Primary: Anti-BAMBI (TMAB-00184) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 29 kDa Observed band size: 30 kDa 4. Sample: RAW264.7 (Mouse) CellLysate at 30 μg Primary: Anti-BAMBI (TMAB-00184) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 27 kDa Observed band size: 30 kDa |
Application | WB,IHC-P,IHC-Fr,IF |
Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Membrane. |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | BAMBI is a membrane spanning glycoprotein that acts as a negative regulator of TGF-β signaling during development. The BAMBI family of proteins are related to type I TGF-β receptor family, however, BAMBI is a pseudoreceptor that lacks an intracellular serine/threonine kinase domain. BAMBI transcription regulation is under the influence of β-catenin, BMP, smad3 and smad4. BAMBI expression can increase in colorectal and hepatocellular carcinomas relative to non-cancerous tissues. BAMBI is expressed at high levels during odontogenesis. BAMBI is coexpressed with Bmp-4 during early Xenopus embryogenesis and can be detected in poorly metastatic human melanoma cell lines. |
Immunogen | KLH conjugated synthetic peptide: human BAMBI |
Antigen Species | Human |
Gene ID | |
Uniprot ID | |
Biology Area | TGF,Surface Molecules |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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