Anti-BAMBI Polyclonal Antibody is a Rabbit antibody targeting BAMBI. Anti-BAMBI Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse (predicted:Rat,Dog,Pig,Cow,Rabbit,Sheep,GuineaPig)

1. Sample:
Testis (Mouse) Lysate at 40 μg
Spleen (Mouse) Lysate at 40 μg
Primary: Anti-BAMBI (TMAB-00184) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 27 kDa
Observed band size: 30 kDa
2. Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-BAMBI Polyclonal Antibody, Unconjugated (TMAB-00184) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Sample:
Lane 1: K562 (Human) Cell Lysate at 30 μg
Lane 2: MCF-7 (Human) Cell Lysate at 30 μg
Lane 3: HepG2 (Human) Cell Lysate at 30 μg
Lane 4: A549 (Human) Cell Lysate at 30 μg
Primary:
Anti-BAMBI (TMAB-00184) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 29 kDa
Observed band size: 30 kDa
4. Sample:
RAW264.7 (Mouse) CellLysate at 30 μg
Primary: Anti-BAMBI (TMAB-00184) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 27 kDa
Observed band size: 30 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human BAMBI

Human

TGF,Surface Molecules