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Anti-BAMBI Polyclonal Antibody

😃Good
Catalog No. TMAB-00184
Alias BMP and activin membrane-bound inhibitor, 2610003H06Rik

Anti-BAMBI Polyclonal Antibody is a Rabbit antibody targeting BAMBI. Anti-BAMBI Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-BAMBI Polyclonal Antibody

Anti-BAMBI Polyclonal Antibody

😃Good
Catalog No. TMAB-00184Alias BMP and activin membrane-bound inhibitor, 2610003H06Rik
Anti-BAMBI Polyclonal Antibody is a Rabbit antibody targeting BAMBI. Anti-BAMBI Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$2217-10 days
100 μL$3737-10 days
200 μL$5297-10 days
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Product Introduction

Bioactivity
Description
Anti-BAMBI Polyclonal Antibody is a Rabbit antibody targeting BAMBI. Anti-BAMBI Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
AliasBMP and activin membrane-bound inhibitor, 2610003H06Rik
Ig Type
IgG
Reactivity
Human,Mouse (predicted:Rat,Dog,Pig,Cow,Rabbit,Sheep,GuineaPig)
Verified Activity
1. Sample:
Testis (Mouse) Lysate at 40 μg
Spleen (Mouse) Lysate at 40 μg
Primary: Anti-BAMBI (TMAB-00184) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 27 kDa
Observed band size: 30 kDa
2. Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-BAMBI Polyclonal Antibody, Unconjugated (TMAB-00184) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Sample:
Lane 1: K562 (Human) Cell Lysate at 30 μg
Lane 2: MCF-7 (Human) Cell Lysate at 30 μg
Lane 3: HepG2 (Human) Cell Lysate at 30 μg
Lane 4: A549 (Human) Cell Lysate at 30 μg
Primary:
Anti-BAMBI (TMAB-00184) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 29 kDa
Observed band size: 30 kDa
4. Sample:
RAW264.7 (Mouse) CellLysate at 30 μg
Primary: Anti-BAMBI (TMAB-00184) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 27 kDa
Observed band size: 30 kDa
Application
WB,IHC-P,IHC-Fr,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationMembrane.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundBAMBI is a membrane spanning glycoprotein that acts as a negative regulator of TGF-β signaling during development. The BAMBI family of proteins are related to type I TGF-β receptor family, however, BAMBI is a pseudoreceptor that lacks an intracellular serine/threonine kinase domain. BAMBI transcription regulation is under the influence of β-catenin, BMP, smad3 and smad4. BAMBI expression can increase in colorectal and hepatocellular carcinomas relative to non-cancerous tissues. BAMBI is expressed at high levels during odontogenesis. BAMBI is coexpressed with Bmp-4 during early Xenopus embryogenesis and can be detected in poorly metastatic human melanoma cell lines.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human BAMBI
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
TGF,Surface Molecules
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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