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Anti-BAMBI Polyclonal Antibody
😃Good
Catalog No. TMAB-00184
Alias BMP and activin membrane-bound inhibitor, 2610003H06Rik
Anti-BAMBI Polyclonal Antibody is a Rabbit antibody targeting BAMBI. Anti-BAMBI Polyclonal Antibody can be used in IF,IHC-Fr,WB.
Anti-BAMBI Polyclonal Antibody
Anti-BAMBI Polyclonal Antibody
😃Good
Catalog No. TMAB-00184Alias BMP and activin membrane-bound inhibitor, 2610003H06Rik
Anti-BAMBI Polyclonal Antibody is a Rabbit antibody targeting BAMBI. Anti-BAMBI Polyclonal Antibody can be used in IF,IHC-Fr,WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $221 | 7-10 days | 7-10 days | |
| 100 μL | $373 | 7-10 days | 7-10 days | |
| 200 μL | $529 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-BAMBI Polyclonal Antibody is a Rabbit antibody targeting BAMBI. Anti-BAMBI Polyclonal Antibody can be used in IF,IHC-Fr,WB. |
| Synonyms | BMP and activin membrane-bound inhibitor, 2610003H06Rik |
| Ig Type | IgG |
| Reactivity | Human,Mouse (predicted:Rat,Dog,Pig,Cow,Rabbit,Sheep,GuineaPig) |
| Verified Activity | 1. Sample: Testis (Mouse) Lysate at 40 μg Spleen (Mouse) Lysate at 40 μg Primary: Anti-BAMBI (TMAB-00184) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 27 kDa Observed band size: 30 kDa 2. Sample: Lane 1: K562 (Human) Cell Lysate at 30 μg Lane 2: MCF-7 (Human) Cell Lysate at 30 μg Lane 3: HepG2 (Human) Cell Lysate at 30 μg Lane 4: A549 (Human) Cell Lysate at 30 μg Primary: Anti-BAMBI (TMAB-00184) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 29 kDa Observed band size: 30 kDa 3. Sample: RAW264.7 (Mouse) CellLysate at 30 μg Primary: Anti-BAMBI (TMAB-00184) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 27 kDa Observed band size: 30 kDa    |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Membrane. |
| Tissue Specificity | High expression in kidney medulla, placenta and spleen; low in kidney cortex, liver, prostate and gut. Not expressed in normal skin, expression is high in melanocytes and in 3 out of 11 melanoma metastases tested. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1 mg/mL |
| Research Background | BAMBI is a membrane spanning glycoprotein that acts as a negative regulator of TGF-β signaling during development. The BAMBI family of proteins are related to type I TGF-β receptor family, however, BAMBI is a pseudoreceptor that lacks an intracellular serine/threonine kinase domain. BAMBI transcription regulation is under the influence of β-catenin, BMP, smad3 and smad4. BAMBI expression can increase in colorectal and hepatocellular carcinomas relative to non-cancerous tissues. BAMBI is expressed at high levels during odontogenesis. BAMBI is coexpressed with Bmp-4 during early Xenopus embryogenesis and can be detected in poorly metastatic human melanoma cell lines. |
| Immunogen | KLH conjugated synthetic peptide: human BAMBI |
| Antigen Species | Human |
| Gene Name | BAMBI |
| Gene ID | |
| Protein Name | BMP and activin membrane-bound inhibitor homolog |
| Uniprot ID | |
| Biology Area | TGF,Surface Molecules |
| Function | Negatively regulates TGF-beta signaling. |
| Molecular Weight | Theoretical: 27 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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