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Anti-BAG3 Antibody (3G694)

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Catalog No. TMAB-03017

Anti-BAG3 Antibody (3G694) is a Rabbit antibody targeting BAG3. Anti-BAG3 Antibody (3G694) can be used in WB,IHC-P,IHC-Fr,IP,IF.

Anti-BAG3 Antibody (3G694)

Anti-BAG3 Antibody (3G694)

Copy Product Info
😃Good
Catalog No. TMAB-03017
Anti-BAG3 Antibody (3G694) is a Rabbit antibody targeting BAG3. Anti-BAG3 Antibody (3G694) can be used in WB,IHC-P,IHC-Fr,IP,IF.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
25 μL$1497-10 days7-10 days
50 μL$2647-10 days7-10 days
100 μL$4727-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-BAG3 Antibody (3G694) is a Rabbit antibody targeting BAG3. Anti-BAG3 Antibody (3G694) can be used in WB,IHC-P,IHC-Fr,IP,IF.
Ig Type
IgG
Clone
3G694
Reactivity
Human
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BAG3 Monoclonal Antibody, Unconjugated (TMAB-03017) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BAG3 Monoclonal Antibody, Unconjugated (TMAB-03017) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
3. 25 μg total protein per lane of various lysates (see on figure) probed with BAG3 monoclonal antibody, unconjugated (TMAB-03017) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; IP: 1:20-50; IF: 1:50-200
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionRecombinant Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation10mM phosphate buffered saline (pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
Research BackgroundCo-chaperone for HSP70 and HSC70 chaperone proteins. Acts as a nucleotide-exchange factor (NEF) promoting the release of ADP from the HSP70 and HSC70 proteins thereby triggering client/substrate protein release.
Antigen Details
Immunogen
A synthesized peptide: human BAG3
Antigen Species
Human
Gene Name
BAG3
Gene ID
Protein Name
BAG family molecular chaperone regulator 3
Uniprot ID
Function
Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Has anti-apoptotic activity.
Chemical Properties
Molecular WeightTheoretical: 62 kDa. Actual: 80 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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