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Anti-Bag1 Antibody (7J25)

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Catalog No. TMAB-03014

Anti-Bag1 Antibody (7J25) is a Rabbit antibody targeting Bag1. Anti-Bag1 Antibody (7J25) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM.

Anti-Bag1 Antibody (7J25)

Anti-Bag1 Antibody (7J25)

😃Good
Catalog No. TMAB-03014
Anti-Bag1 Antibody (7J25) is a Rabbit antibody targeting Bag1. Anti-Bag1 Antibody (7J25) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
25 μL$1487-10 days7-10 days
50 μL$2637-10 days7-10 days
100 μL$4727-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-Bag1 Antibody (7J25) is a Rabbit antibody targeting Bag1. Anti-Bag1 Antibody (7J25) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM.
Ig Type
IgG
Clone
7J25
Reactivity
Human (predicted:Mouse,Rat)
Application
Recommended Dose
WB: 1:500-1000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; ICC/IF: 1:50-200; IF: 1:50-200; FCM: 1:50-100
Antibody Type
Monoclonal
Host SpeciesRabbit
Subcellular LocalizationCytoplasm. Nucleus. Isoform 2 localizes to the cytoplasm and shuttles into the nucleus in response to heat shock; Nucleus. Cytoplasm. Isoform 1 localizes predominantly to the nucleus and Cytoplasm. Nucleus. Isoform 4 localizes predominantly to the cytoplasm. The cellular background in which it is expressed can influence whether it resides primarily in the cytoplasm or is also found in the nucleus. In the presence of BCL2, localizes to intracellular membranes (what appears to be the nuclear envelope and perinuclear membranes) as well as punctate cytosolic structures suggestive of mitochondria.
Tissue SpecificityIsoform 4 is the most abundantly expressed isoform. It is ubiquitously expressed throughout most tissues, except the liver, colon, breast and uterine myometrium. Isoform 1 is expressed in the ovary and testis. Isoform 4 is expressed in several types of tumor cell lines, and at consistently high levels in leukemia and lymphoma cell lines. Isoform 1 is expressed in the prostate, breast and leukemia cell lines. Isoform 3 is the least abundant isoform in tumor cell lines.
ConstructionRecombinant Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation10mM phosphate buffered saline (pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
Research BackgroundCo-chaperone for HSP70 and HSC70 chaperone proteins. Acts as a nucleotide-exchange factor (NEF) promoting the release of ADP from the HSP70 and HSC70 proteins thereby triggering client/substrate protein release. Nucleotide release is mediated via its binding to the nucleotide-binding domain (NBD) of HSPA8/HSC70 where as the substrate release is mediated via its binding to the substrate-binding domain (SBD) of HSPA8/HSC70.
Antigen Details
Immunogen
A synthesized peptide: human BAG1
Antigen Species
Human
Gene Name
BAG1
Gene ID
Protein Name
BAG family molecular chaperone regulator 1
Uniprot ID
Function
Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Inhibits the pro-apoptotic function of PPP1R15A, and has anti-apoptotic activity. Markedly increases the anti-cell death function of BCL2 induced by various stimuli.
Chemical Properties
Molecular WeightTheoretical: 39 kDa. Actual: 33, 46, 50 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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