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Anti-BAF57 Antibody (2O20) is a Rabbit antibody targeting BAF57. Anti-BAF57 Antibody (2O20) can be used in WB,ICC,IF,IHC,IP.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $296 | 7-10 days | |
100 μL | $498 | 7-10 days |
Description | Anti-BAF57 Antibody (2O20) is a Rabbit antibody targeting BAF57. Anti-BAF57 Antibody (2O20) can be used in WB,ICC,IF,IHC,IP. |
Synonyms | SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1, SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily e member 1, SWI/SNF related matrix associated actin dependent regulator of chromatin e1, SMCE1_HUMAN, SMARCE1, SMARCE 1, FLJ35648, Chromatin remodeling complex BRG1 associated factor 57, BRG1-associated factor 57, BRG1 associated factor 57, BAF 57 |
Ig Type | IgG |
Reactivity | Human,Mouse,Rat |
Verified Activity | 1. Western blot analysis of BAF57 on SH-SY-5Y cell lysate using anti-BAF57 antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded rat uterus tissue using anti-BAF57 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human gallbladder tissue using anti-BAF57 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-BAF57/SMARCE1 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-BAF57 antibody. Counter stained with hematoxylin. 6. ICC staining BAF57 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining BAF57 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. ICC staining BAF57 in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
Application | |
Recommended Dose | WB: 1:500-2000; IHC: 1:50-200; ICC:IF: 1:50-200; IP: 1:50-200 |
Antibody Type | Monoclonal |
Host Species | Rabbit |
Construction | Recombinant Antibody |
Purification | ProA affinity purified |
Appearance | Liquid |
Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
Research Background | The SWI/SNF complex is involved in the activation of transcription via the remodeling of nucleosome structure in an ATP-dependent manner. Brm (also designated SNF1 or SNF2α) and Brg-1 (also designated SNF2 or SNF2β) are the ATPase subunits of the mammalian SWI/SNF complex. Brm, Brg-1, Ini1 (integrase interactor 1, also designated SNF5), BAF155 (also designated SRG3) and BAF170 are thought to comprise the functional core of the SWI/SNF complex. In higher eukaryotes, BAF57 is also a critical component of the SWI/ SNF complex. BAF57 contains a high-mobility-group (HMG) domain adjacent to a kinesin-like region and is a DNA-binding subunit of the SWI/SNF complex. The human BAF57 gene maps within the q12-25 region of chromosome 17, a gene-rich area implicated in breast and ovarian cancers. |
Conjucates | Unconjugated |
Immunogen | Recombinant Protein: human BAF57 aa 1-200 |
Antigen Species | human |
Uniprot ID |
Molecular Weight | Theoretical: 47 kDa. |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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