Shopping Cart
  • Remove All
  • TargetMol
    Your shopping cart is currently empty

Anti-BACE1 Polyclonal Antibody

😃Good
Catalog No. TMAB-00180
Alias β-site APP-cleaving enzyme 1, HSPC104, beta-site APP-cleaving enzyme 1, BACE, ASP2

Anti-BACE1 Polyclonal Antibody is a Rabbit antibody targeting BACE1. Anti-BACE1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.

Anti-BACE1 Polyclonal Antibody

Anti-BACE1 Polyclonal Antibody

😃Good
Catalog No. TMAB-00180Alias β-site APP-cleaving enzyme 1, HSPC104, beta-site APP-cleaving enzyme 1, BACE, ASP2
Anti-BACE1 Polyclonal Antibody is a Rabbit antibody targeting BACE1. Anti-BACE1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
Pack SizePriceAvailabilityQuantity
50 μL$2207-10 days
100 μL$3747-10 days
200 μL$5287-10 days
Add to Cart
Add to Quotation
Bulk & Custom
Questions
View More
Contact us for more batch information
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

Product Introduction

Bioactivity
Description
Anti-BACE1 Polyclonal Antibody is a Rabbit antibody targeting BACE1. Anti-BACE1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
Aliasβ-site APP-cleaving enzyme 1, HSPC104, beta-site APP-cleaving enzyme 1, BACE, ASP2
Ig Type
IgG
Reactivity
Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Rabbit,GuineaPig)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (BACE1) Polyclonal Antibody, Unconjugated (TMAB-00180) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (BACE1) Polyclonal Antibody, Unconjugated (TMAB-00180) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
3. Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (BACE1) polyclonal Antibody, Unconjugated (TMAB-00180) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.
4. Sample:
Lane 1: SH-SY5Y (Human) Cell Lysate at 30 μg
Lane 2: Huvec (Human) Cell Lysate at 30 μg
Primary: Anti-BACE1 (TMAB-00180) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60-70 kDa
Observed band size: 68 kDa
Application
WB,IHC-P,IHC-Fr,ICC/IF,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationMembrane.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundCerebral deposition of amyloid beta peptide is an early and critical feature of Alzheimer's disease. Amyloid beta peptide is generated by proteolytic cleavage of amyloid precursor protein(APP) by two proteases, one of which is the protein encoded by this gene. The encoded protein, a member of the peptidase A1 protein family, is a type I integral membrane glycoprotein and aspartic protease that is found mainly in the Golgi. Multiple transcript variants encoding different isoforms have been described for this gene.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human BACE1
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
BACEs,Proteases
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

Calculator

  • Molarity Calculator
  • Dilution Calculator
  • Reconstitution Calculator
  • Molecular Weight Calculator

Sci Citations

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

Related Tags: buy Anti-BACE1 Polyclonal Antibody | purchase Anti-BACE1 Polyclonal Antibody | Anti-BACE1 Polyclonal Antibody cost | order Anti-BACE1 Polyclonal Antibody