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Anti-BACE1 Antibody (8D358)
😃Good
Catalog No. TMAY-00206
Alias β-site APP-cleaving enzyme 1, HSPC104, beta-site APP-cleaving enzyme 1, BACE, ASP2
Anti-BACE1 Antibody (8D358) is a Rabbit antibody targeting BACE1. Anti-BACE1 Antibody (8D358) can be used in FCM,ICC/IF.
Anti-BACE1 Antibody (8D358)
Anti-BACE1 Antibody (8D358)
😃Good
Catalog No. TMAY-00206Alias β-site APP-cleaving enzyme 1, HSPC104, beta-site APP-cleaving enzyme 1, BACE, ASP2
Anti-BACE1 Antibody (8D358) is a Rabbit antibody targeting BACE1. Anti-BACE1 Antibody (8D358) can be used in FCM,ICC/IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $268 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Monoclonal Application: FCM, ICC/IF Reactivity: Human |
| Alias | β-site APP-cleaving enzyme 1, HSPC104, beta-site APP-cleaving enzyme 1, BACE, ASP2 |
| Ig Type | Rabbit IgG |
| Clone | 8D358 |
| Reactivity | Human |
| Verified Activity | 1. Immunofluorescence staining of Human BACE1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human AK4 monoclonal antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm. 2. Flow cytometric analysis of Human BACE1 expression on Jurkat cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human BACE1, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. |
| Application | FCM,ICC/IF |
| Recommended Dose | ICC-IF: 1:20-1:100; FCM: 1:25-1:100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Human BACE1 (rh BACE1; TMPY-00752; NP_036236.1; Met1-Thr457). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Beta-site APP-cleaving enzyme 1 (BACE1) is an aspartic-acid protease important in the formation of myelin sheaths in peripheral nerve cells. In the brain, This protein is expressed highly in the substantia nigra, locus coruleus and medulla oblongata. Strong BACE1 expression has also been described in pancreatic tissue. BACE1 has a pivotal role in the pathogenesis of Alzheimer's disease. In Alzheimer's disease patients, BACE1 levels were elevated although mRNA levels were not changed. It has been found that BACE1 gene expression is controlled by a TATA-less promoter. The translational repression as a new mechanism controlling its expression. And the low concentrations of Ca(2+) (microM range) significantly increased the proteolytic activity of BACE1. Furthermore, BACE1 protein is ubiquitinated, and the degradation of BACE1 proteins and amyloid precursor protein processing are regulated by the ubiquitin-proteasome pathway. It has also been identified as the rate limiting enzyme for amyloid-beta-peptide (Abeta) production. |
| Conjucates | Unconjugated |
| Others Formats | FITC/PE |
| Antibody Types Available | 3 |
| Immunogen | Recombinant Protein: Human BACE1 protein (TMPY-00752) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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