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Anti-B2M/beta 2-Microglobulin Antibody (8W936)

🥰Excellent
Catalog No. TMAY-02176

Anti-B2M/beta 2-Microglobulin Antibody (8W936) is a Mouse antibody targeting B2M/beta 2-Microglobulin. Anti-B2M/beta 2-Microglobulin Antibody (8W936) can be used in FCM,ICC/IF.

Anti-B2M/beta 2-Microglobulin Antibody (8W936)

Anti-B2M/beta 2-Microglobulin Antibody (8W936)

🥰Excellent
Catalog No. TMAY-02176
Anti-B2M/beta 2-Microglobulin Antibody (8W936) is a Mouse antibody targeting B2M/beta 2-Microglobulin. Anti-B2M/beta 2-Microglobulin Antibody (8W936) can be used in FCM,ICC/IF.
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Product Introduction

Bioactivity
Description
Anti-B2M/beta 2-Microglobulin Antibody (8W936) is a Mouse antibody targeting B2M/beta 2-Microglobulin. Anti-B2M/beta 2-Microglobulin Antibody (8W936) can be used in FCM,ICC/IF.
Ig Type
Monoclonal Mouse IgG1
Clone
8W936
Reactivity
Human
Verified Activity
1. Immunofluorescence staining of Human B2M in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with mouse anti-Human B2M monoclonal antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.

2. Flow cytometric analysis of Human β2M, expression on human whole blood Lymphocytes. Cells were stained with purified anti-Human β2M, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes.
Application
FCM,ICC/IF
Recommended Dose
ICC-IF: 1:20-1:100; FCM: 1:25-1:100
Antibody Type
Monoclonal
Host SpeciesMouse
ConstructionThis antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human B2M / beta-2 microglobulin (rh B2M / beta-2 microglobulin; TMPY-01686; NP_004039.1; Met1-Met119). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundB2M, also known as β2-Microglobulin or CDABP0092, is a component of MHC class I molecules found expression in all nucleated cells (excludes red blood cells). The major function of MHC class I moleculesis is to display fragments of proteins from within the cell to T-cells and cells containing foreign proteins will be attacked. B2M (β2-Microglobulin) is a low molecular weight protein. It was demonstrated that B2M (β2-Microglobulin) was localized in the membranes of nucleated cells and was found to be associated with HL-A antigens. B2M (β2- Microglobulin) is present in free form in various body fluids and as a subunit of histocompatibility antigens on cell surfaces lateral to the α3 chain. Unlike α3, β2 has no transmembrane region. Directly above β2 lies the α1 chain, which itself is lateral to the α2. In the absence of B2M (β2 microglobulin), very limited amounts of MHC class I (classical and non-classical) molecules can be detected on the surface. In the absence of MHC class I, CD8 T cells, a subset of T cells involved in the development of acquired immunity cannot develop. Low levels of B2M (β2 microglobulin) can indicate non-progression of HIV.
Related Conjugates and Formulations
Conjucates
Unconjugated
Others Formats
FITC/PE
Antibody Types Available
3
Antigen Details
Immunogen
Recombinant Human B2M / beta-2 microglobulin Protein (TMPY-01686)
Antigen Species
Human
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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