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Anti-AXL Polyclonal Antibody
😃Good
Catalog No. TMAB-00177
Anti-AXL Polyclonal Antibody is a Rabbit antibody targeting AXL. Anti-AXL Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-AXL Polyclonal Antibody
Anti-AXL Polyclonal Antibody
😃Good
Catalog No. TMAB-00177
Anti-AXL Polyclonal Antibody is a Rabbit antibody targeting AXL. Anti-AXL Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-AXL Polyclonal Antibody is a Rabbit antibody targeting AXL. Anti-AXL Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Mouse (predicted:Rat,Dog,Horse,Rabbit) |
| Verified Activity | 1. Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-AXL/UFO Polyclonal Antibody, Unconjugated (TMAB-00177) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 2. Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-AXL/UFO Polyclonal Antibody, Unconjugated (TMAB-00177) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 3. Sample: MKN45 (Human) Cell Lysate at 30 μg SW480 (Human) Cell Lysate at 30 μg FHc (Human) Cell Lysate at 30 μg Primary: Anti-AXL (TMAB-00177) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 140/120/98 kDa Observed band size: 120 kDa 4. Sample: Testis (Mouse) Lysate at 40 μg Hela (Human) Cell Lysate at 30 μg Primary: Anti-AXL (TMAB-00177) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 140/120/65 kDa Observed band size: 120 kDa 5. Sample: LOVO (Human) Cell Lysate at 40 μg K562 (Human) Cell Lysate at 40 μg Primary: Anti-AXL (TMAB-00177) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 95 kDa Observed band size: 95 kDa |
| Application | WB,IHC-P,IHC-Fr,IF |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cell membrane; Single-pass type I membrane protein. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | The protein encoded by this gene is a member of the receptor tyrosine kinase subfamily. Although it is similar to other receptor tyrosine kinases, the Axl protein represents a unique structure of the extracellular region that juxtaposes IgL and FNIII repeats. It transduces signals from the extracellular matrix into the cytoplasm by binding growth factors such as vitamin K dependent protein growth arrest specific gene 6. It is involved in the stimulation of cell proliferation. This receptor can also mediate cell aggregation by homophilic binding. Axl is a chronic myelogenous leukemia associated oncogene and also associated with colon cancer and melanoma.The Axl gene is evolutionarily conserved between vertebrate species. This gene has two different alternatively spliced transcript variants (AXL1 and AXL2). |
| Immunogen | KLH conjugated synthetic peptide: human AXL |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Kinases/phosphatases,Signal transducers,Signal transduction,Kinases/Phosphatases,Receptor Tyrosine Kinases,Tumor Associated |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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