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Anti-AURKA Antibody (1Y815)
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Catalog No. TMAC-00349
Alias AYK 1
Anti-AURKA Antibody (1Y815) is a Rabbit antibody targeting AURKA. Anti-AURKA Antibody (1Y815) can be used in WB,ICC/IF,FCM.
Anti-AURKA Antibody (1Y815)
Anti-AURKA Antibody (1Y815)
😃Good
Catalog No. TMAC-00349Alias AYK 1
Anti-AURKA Antibody (1Y815) is a Rabbit antibody targeting AURKA. Anti-AURKA Antibody (1Y815) can be used in WB,ICC/IF,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $297 | 7-10 days | |
| 100 μL | $496 | 7-10 days |
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Product Introduction
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Antigen Details
Chemical Properties
| Description | Anti-AURKA Antibody (1Y815) is a Rabbit antibody targeting AURKA. Anti-AURKA Antibody (1Y815) can be used in WB,ICC/IF,FCM. |
| Synonyms | AYK 1 |
| Ig Type | IgG |
| Clone | 1Y815 |
| Reactivity | Human,Mouse |
| Verified Activity | 1. ICC staining Aurora A in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 2. ICC staining Aurora A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 3. ICC staining Aurora A in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. Flow cytometric analysis of Hela cells with Aurora A antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:1000-2000; ICC/IF: 1:100-500; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Activation of the oncogenic protein kinase Aurora A regulates meiotic and mitotic cell cycles in eukaryotic cells. Specifically, Aurora A plays a key role in G2/M progression. Activation occurs via autophosphorylation, and while 14 sites are subject to this, only the threonine residue at position 295 is required for activity. Though autophosphorylation mediates activation, a number of other proteins influence activation, including the spindle assembly factor TPX2 and p53 |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 46 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
Keywords
STK6STK15STK 6STK 15Serine/threonine-protein kinase Ayk1Serine/threonine-protein kinase aurora-ASerine/threonine-protein kinase 6Serine/threonine-protein kinase 15Ipl1- and aurora-related kinase 1IAK1IAK 1BTAKBreast tumor-amplified kinaseAYK1Aurora-related kinase 1)Aurora2Aurora/IPL1-related kinase 1 (ARK-1Aurora kinase-AAurora kinase AAurora 2AURKAAURAARK-1ARK1ARK 1AIRK1AIRK 1AIK
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