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Anti-AURKA Antibody (1Y815) (Synonyms: AYK 1)

Catalog No. TMAC-00349 Copy Product Info
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Anti-AURKA Antibody (1Y815) is a Rabbit antibody targeting AURKA. Anti-AURKA Antibody (1Y815) can be used in FCM,ICC/IF,WB.

Anti-AURKA Antibody (1Y815)

Copy Product Info
🥰Excellent
Catalog No. TMAC-00349
Synonyms AYK 1

Anti-AURKA Antibody (1Y815) is a Rabbit antibody targeting AURKA. Anti-AURKA Antibody (1Y815) can be used in FCM,ICC/IF,WB.

Anti-AURKA Antibody
(1Y815)
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2977-10 days7-10 days
100 μL$4967-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-AURKA Antibody (1Y815) is a Rabbit antibody targeting AURKA. Anti-AURKA Antibody (1Y815) can be used in FCM,ICC/IF,WB.
SynonymsAYK 1
Ig Type
IgG
Clone
1Y815
Reactivity
Human,Mouse
Verified Activity
1. ICC staining Aurora A in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
2. ICC staining Aurora A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
3. ICC staining Aurora A in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
4. Flow cytometric analysis of Hela cells with Aurora A antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as secondary antibody.
verifiedActivityverifiedActivityverifiedActivityverifiedActivity
Application
Recommended Dose
WB: 1:1000-2000; ICC/IF: 1:100-500; FCM: 1:50-100
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionRecombinant Antibody
PurificationProA affinity purified
AppearanceLiquid
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Research BackgroundActivation of the oncogenic protein kinase Aurora A regulates meiotic and mitotic cell cycles in eukaryotic cells. Specifically, Aurora A plays a key role in G2/M progression. Activation occurs via autophosphorylation, and while 14 sites are subject to this, only the threonine residue at position 295 is required for activity. Though autophosphorylation mediates activation, a number of other proteins influence activation, including the spindle assembly factor TPX2 and p53
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 46 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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