Anti-ATXN10 Polyclonal Antibody is a Rabbit antibody targeting ATXN10. Anti-ATXN10 Polyclonal Antibody can be used in WB,FCM.

IgG

Mouse,Rat (predicted:Human,Dog,Pig,Cow,Horse,Sheep)

1. Blank control: Mouse spleen.
Primary Antibody (green line): Rabbit Anti-ATXN10 antibody (TMAB-02967)
Dilution: 1 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1 μg /test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
2. Sample:
Brain (Rat) Lysate at 40 μg
Cerebrum (Mouse) Lysate at 40 μg
Primary: Anti-ATXN10 (TMAB-02967) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53 kD
Observed band size: 52 kD
3. Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Mouse Cerebellum tissue lysates
Lane 3: Rat Cerebrum tissue lysates
Lane 4: Rat Cerebellum tissue lysates
Primary: Anti-ATXN10 (TMAB-02967) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53 kDa
Observed band size: 52 kDa

WB: 1:500-2000; FCM: 2μg/Test

Polyclonal

KLH conjugated synthetic peptide: human ATXN10/SCA10

Human

ATXN10

Ataxin-10

Necessary for the survival of cerebellar neurons. Induces neuritogenesis by activating the Ras-MAP kinase pathway. May play a role in the maintenance of a critical intracellular glycosylation level and homeostasis.