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Anti-Atrial natriuretic factor Polyclonal Antibody is a Rabbit antibody targeting Atrial natriuretic factor. Anti-Atrial natriuretic factor Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $222 | 7-10 days | |
100 μL | $372 | 7-10 days | |
200 μL | $529 | 7-10 days |
Description | Anti-Atrial natriuretic factor Polyclonal Antibody is a Rabbit antibody targeting Atrial natriuretic factor. Anti-Atrial natriuretic factor Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
Alias | Prepronatriodilatin, PND, Nppa, Natriuretic peptides A, CDP, CDD-ANF, CDD ANF, Cardiodilatin, Atrial natriuretic peptide, Atrial natriuretic factor, ANP, ANF |
Ig Type | IgG |
Reactivity | Human,Mouse,Rat (predicted:Dog,Pig,Cow,Horse,Rabbit,Sheep,GuineaPig) |
Verified Activity | 1. Sample: Heart (Mouse) Lysate at 40 μg Heart (Rat) Lysate at 40 μg Primary: Anti-Atrial natriuretic factor (TMAB-00172) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 3/17 kDa Observed band size: 15 kDa 2. Paraformaldehyde-fixed, paraffin embedded (Rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Atrial natriuretic factor) Polyclonal Antibody, Unconjugated (TMAB-00172) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
Application | WB,IHC-P,IHC-Fr,IF |
Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Secreted. |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | The protein encoded by this gene belongs to the natriuretic peptide family. Natriuretic peptides are implicated in the control of extracellular fluid volume and electrolyte homeostasis. This protein is synthesized as a large precursor (containing a signal peptide), which is processed to release a peptide from the N-terminus with similarity to vasoactive peptide, cardiodilatin, and another peptide from the C-terminus with natriuretic-diuretic activity. Mutations in this gene have been associated with atrial fibrillation familial type 6. [provided by RefSeq, Sep 2009].. |
Immunogen | KLH conjugated synthetic peptide: human ANP |
Antigen Species | Human |
Gene ID | |
Uniprot ID | |
Biology Area | Blood Pressure regulation,Blood,Hormones,Hypertrophy,Hormones |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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