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Anti-ATPIF1 Polyclonal Antibody

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Catalog No. TMAB-02959

Anti-ATPIF1 Polyclonal Antibody is a Rabbit antibody targeting ATPIF1. Anti-ATPIF1 Polyclonal Antibody can be used in WB.

Anti-ATPIF1 Polyclonal Antibody

Anti-ATPIF1 Polyclonal Antibody

😃Good
Catalog No. TMAB-02959
Anti-ATPIF1 Polyclonal Antibody is a Rabbit antibody targeting ATPIF1. Anti-ATPIF1 Polyclonal Antibody can be used in WB.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
50 μL$2237-10 days7-10 days
100 μL$3737-10 days7-10 days
200 μL$5287-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-ATPIF1 Polyclonal Antibody is a Rabbit antibody targeting ATPIF1. Anti-ATPIF1 Polyclonal Antibody can be used in WB.
Ig Type
IgG
Reactivity
Human (predicted:Mouse,Rat,Dog,Horse,Rabbit)
Application
WB
Recommended Dose
WB: 1:500-2000
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationMitochondrion.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundMitochondrial ATP synthases (ATPases) transduce the energy contained in membrane electrochemical proton gradients into the energy required for synthesis of high-energy phosphate bonds. ATPases contain two linked complexes: F1, the hydrophilic catalytic core; and F0, the membrane-embedded protein channel. F1 consists of three Alpha chains and three Beta chains, which are weakly homologous, as well as one Gamma chain, one Delta chain and one Gamma chain. F0 consists of three subunits: a, b and c. A mitochondrial F1-ATPase inhibitor protein, ATPIF1 (ATPase inhibitory factor 1), also known as IP, IF1, ATPI or ATPIP (ATPase inhibitor protein), binds to the C-terminal region of a Beta subunit of the F1-ATPase at low pH values and, via interference of the Beta and Gamma subunit interaction, ATPIF1 regulates the activity of the F1F0-ATPase. This reversible ATPIF1 binding to F1F0-ATPase also occurs on the surface of endothelial cells.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human ATPIF1/ATPase Inhibitory Factor 1
Antigen Species
Human
Gene Name
ATP5IF1
Gene ID
93974
Protein Name
ATPase inhibitor, mitochondrial
Uniprot ID
Q9UII2
Function
Endogenous F(1)F(o)-ATPase inhibitor limiting ATP depletion when the mitochondrial membrane potential falls below a threshold and the F(1)F(o)-ATP synthase starts hydrolyzing ATP to pump protons out of the mitochondrial matrix.
Chemical Properties
Molecular WeightTheoretical: 10 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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