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Anti-ATP6V0D2 Polyclonal Antibody
😃Good
Catalog No. TMAB-00169
Anti-ATP6V0D2 Polyclonal Antibody is a Rabbit antibody targeting ATP6V0D2. Anti-ATP6V0D2 Polyclonal Antibody can be used in WB.
Anti-ATP6V0D2 Polyclonal Antibody
Anti-ATP6V0D2 Polyclonal Antibody
😃Good
Catalog No. TMAB-00169
Anti-ATP6V0D2 Polyclonal Antibody is a Rabbit antibody targeting ATP6V0D2. Anti-ATP6V0D2 Polyclonal Antibody can be used in WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $374 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-ATP6V0D2 Polyclonal Antibody is a Rabbit antibody targeting ATP6V0D2. Anti-ATP6V0D2 Polyclonal Antibody can be used in WB. |
| Ig Type | IgG |
| Reactivity | Human (predicted:Mouse,Rat,Dog,Pig,Sheep) |
| Verified Activity | Sample: Lane 1: Human HeLa cell lysates Lane 2: Human A549 cell lysates Primary: Anti-ATP6V0D2 (TMAB-00169) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 40 kDa Observed band size: 40 kDa |
| Application | WB |
| Recommended Dose | WB: 1:500-2000 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Apical plasma membrane. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | Vacuolar-type H+-ATPase (V-ATPase) is a multisubunit enzyme responsible for acidification of eukaryotic intracellular organelles. V-ATPases pump protons against an electrochemical gradient, while F-ATPases reverse the process, thereby synthesizing ATP. A peripheral V1 domain, which is responsible for ATP hydrolysis, and a integral V0 domain, which is responsible for proton translocation, compose V-ATPase. Nine subunits (A–H) make up the V1 domain and five subunits (a, d, c, c' and c") make up the V0 domain. Like F-ATPase, V-ATPase most likely operates through a rotary mechanism. V-ATPase D2 is a 350 amino acid protein that is expressed in kidney, lung and osteoclast. V-ATPase D2 has been implicated as a regulator of urine acidification, osteoclast fusion and bone formation. Furthermore, V-ATPase D2 has been identified as a dendritic cell marker. |
| Immunogen | KLH conjugated synthetic peptide: human ATP6V0D2/V-ATPase D2 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | ATPases |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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