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Anti-ATG4C Polyclonal Antibody

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Catalog No. TMAB-02920

Anti-ATG4C Polyclonal Antibody is a Rabbit antibody targeting ATG4C. Anti-ATG4C Polyclonal Antibody can be used in WB.

Anti-ATG4C Polyclonal Antibody

Anti-ATG4C Polyclonal Antibody

Copy Product Info
😃Good
Catalog No. TMAB-02920
Anti-ATG4C Polyclonal Antibody is a Rabbit antibody targeting ATG4C. Anti-ATG4C Polyclonal Antibody can be used in WB.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
50 μL$2237-10 days7-10 days
100 μL$3737-10 days7-10 days
200 μL$5277-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-ATG4C Polyclonal Antibody is a Rabbit antibody targeting ATG4C. Anti-ATG4C Polyclonal Antibody can be used in WB.
Ig Type
IgG
Reactivity
Human,Mouse,Rat (predicted:Dog,Pig,Cow,Horse,Rabbit)
Verified Activity
1. Sample:
A673 (Human) Cell Lysate at 30 μg
Primary: Anti-ATG4C (TMAB-02920) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kD
Observed band size: 55 kD
2. Sample:
Liver (Mouse) Lysate at 40 μg
Primary: Anti-ATG4C (TMAB-02920) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kD
Observed band size: 55 kD
3. Sample:
Lane 1: Mouse Muscle tissue lysates
Lane 2: Mouse Heart tissue lysates
Lane 3: Rat Muscle tissue lysates
Primary: Anti-ATG4C (TMAB-02920) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa
Application
Recommended Dose
WB: 1:500-2000
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCytoplasm (Probable).
Tissue SpecificityHighly expressed in skeletal muscle, heart, liver and testis.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundCysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human ATG4C
Antigen Species
Human
Gene Name
ATG4C
Gene ID
Protein Name
Cysteine protease ATG4C
Uniprot ID
Function
Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.
Chemical Properties
Molecular WeightTheoretical: 52 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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