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Anti-ATG3 Antibody (2X547)

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Catalog No. TMAB-02917

Anti-ATG3 Antibody (2X547) is a Rabbit antibody targeting ATG3. Anti-ATG3 Antibody (2X547) can be used in WB,ICC/IF.

Anti-ATG3 Antibody (2X547)

Anti-ATG3 Antibody (2X547)

😃Good
Catalog No. TMAB-02917
Anti-ATG3 Antibody (2X547) is a Rabbit antibody targeting ATG3. Anti-ATG3 Antibody (2X547) can be used in WB,ICC/IF.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
25 μL$1497-10 days7-10 days
50 μL$2647-10 days7-10 days
100 μL$4707-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-ATG3 Antibody (2X547) is a Rabbit antibody targeting ATG3. Anti-ATG3 Antibody (2X547) can be used in WB,ICC/IF.
Ig Type
IgG
Clone
2X547
Reactivity
Human,Mouse,Rat
Application
Recommended Dose
WB: 1:500-2000; ICC/IF: 1:50-200
Antibody Type
Monoclonal
Host SpeciesRabbit
Subcellular LocalizationCytoplasm.
Tissue SpecificityWidely expressed, with a highest expression in heart, skeletal muscle, kidney, liver and placenta.
ConstructionRecombinant Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation10mM phosphate buffered saline (pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
Research BackgroundE2 conjugating enzyme that catalyzes the covalent conjugation of the C-terminal Gly of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A) to the amino group of phosphatidylethanolamine (PE)-containing lipids in the membrane resulting in membrane-bound ATG8-like proteins which is one of the key steps in the development of autophagic isolation membranes during autophagosome formation.
Antigen Details
Immunogen
A synthesized peptide: human ATG3
Antigen Species
Human
Gene Name
ATG3
Gene ID
Protein Name
Ubiquitin-like-conjugating enzyme ATG3
Uniprot ID
Function
E2-like enzyme involved in autophagy and mitochondrial homeostasis. Catalyzes the conjugation of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A) to phosphatidylethanolamine (PE). PE-conjugation to ATG8-like proteins is essential for autophagy. Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3.
Chemical Properties
Molecular WeightTheoretical: 36 kDa. Actual: 40 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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