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Anti-ATG3 Antibody (2X547)
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Catalog No. TMAB-02917
Anti-ATG3 Antibody (2X547) is a Rabbit antibody targeting ATG3. Anti-ATG3 Antibody (2X547) can be used in WB,ICC/IF.
Anti-ATG3 Antibody (2X547)
Anti-ATG3 Antibody (2X547)
Copy Product Info😃Good
Catalog No. TMAB-02917
Anti-ATG3 Antibody (2X547) is a Rabbit antibody targeting ATG3. Anti-ATG3 Antibody (2X547) can be used in WB,ICC/IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 25 μL | $149 | 7-10 days | 7-10 days | |
| 50 μL | $264 | 7-10 days | 7-10 days | |
| 100 μL | $470 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-ATG3 Antibody (2X547) is a Rabbit antibody targeting ATG3. Anti-ATG3 Antibody (2X547) can be used in WB,ICC/IF. |
| Ig Type | IgG |
| Clone | 2X547 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 25 μg total protein per lane of various lysates (see on figure) probed with ATG3 monoclonal antibody, unconjugated (TMAB-02917) at 1: 2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min. |
| Application | |
| Recommended Dose | WB: 1:500-2000; ICC/IF: 1:50-200 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. |
| Tissue Specificity | Widely expressed, with a highest expression in heart, skeletal muscle, kidney, liver and placenta. |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 10mM phosphate buffered saline (pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| Research Background | E2 conjugating enzyme that catalyzes the covalent conjugation of the C-terminal Gly of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A) to the amino group of phosphatidylethanolamine (PE)-containing lipids in the membrane resulting in membrane-bound ATG8-like proteins which is one of the key steps in the development of autophagic isolation membranes during autophagosome formation. |
| Immunogen | A synthesized peptide: human ATG3 |
| Antigen Species | Human |
| Gene Name | ATG3 |
| Gene ID | |
| Protein Name | Ubiquitin-like-conjugating enzyme ATG3 |
| Uniprot ID | |
| Function | E2-like enzyme involved in autophagy and mitochondrial homeostasis. Catalyzes the conjugation of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A) to phosphatidylethanolamine (PE). PE-conjugation to ATG8-like proteins is essential for autophagy. Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3. |
| Molecular Weight | Theoretical: 36 kDa. Actual: 40 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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