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Anti-ATG18 Antibody (8B338)
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Catalog No. TMAB-02915
Anti-ATG18 Antibody (8B338) is a Rabbit antibody targeting ATG18. Anti-ATG18 Antibody (8B338) can be used in FCM, IF, IHC-Fr, IHC-P, WB.
Anti-ATG18 Antibody (8B338)
Anti-ATG18 Antibody (8B338)
😃Good
Catalog No. TMAB-02915
Anti-ATG18 Antibody (8B338) is a Rabbit antibody targeting ATG18. Anti-ATG18 Antibody (8B338) can be used in FCM, IF, IHC-Fr, IHC-P, WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 25 μL | $151 | 7-10 days | 7-10 days | |
| 50 μL | $262 | 7-10 days | 7-10 days | |
| 100 μL | $470 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-ATG18 Antibody (8B338) is a Rabbit antibody targeting ATG18. Anti-ATG18 Antibody (8B338) can be used in FCM, IF, IHC-Fr, IHC-P, WB. |
| Ig Type | IgG |
| Clone | 8B338 |
| Reactivity | Human,Mouse,Rat |
| Application | |
| Recommended Dose | FCM=1:50-100; IF=1:100-200; IHC-Fr=1:100-200; IHC-P=1:100-200; WB=1:500-2000 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Golgi apparatus, trans-Golgi network. Endosome. Cytoplasmic vesicle, clathrin-coated vesicle. Preautophagosomal structure membrane; Peripheral membrane protein. Cytoplasm, cytoskeleton. Note=Trans elements of the Golgi and peripheral endosomes. Dynamically cycles through these compartments and is susceptible to conditions that modulate membrane flux. Enriched in clathrin-coated vesicles. Upon starvation-induced autophagy, accumulates at subcellular structures in the cytoplasm: enlarged vesicular and lasso-like structures, and large cup-shaped structures predominantly around the nucleus. Recruitment to autophagic membranes is controlled by MTMR14. Labile microtubules specifically recruit markers of autophagosome formation like WIPI1, whereas mature autophagosomes may bind to stable microtubules. |
| Tissue Specificity | Ubiquitously expressed. Highly expressed in skeletal muscle, heart, testis, pancreas and placenta. Highly expressed in G361, Sk-mel-28, Sk-mel-13, WM852 and WM451 cells. Up-regulated in a variety of tumor tissues. |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 10mM phosphate buffered saline (pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| Research Background | Component of the autophagy machinery that controls the major intracellular degradation process by which cytoplasmic materials are packaged into autophagosomes and delivered to lysosomes for degradation. |
| Immunogen | A synthesized peptide: human WIPI 1 |
| Antigen Species | Human |
| Gene Name | WIPI1 |
| Gene ID | |
| Protein Name | WD repeat domain phosphoinositide-interacting protein 1 |
| Uniprot ID | |
| Function | Plays an important role in autophagy and in particular starvation- and calcium-mediated autophagy, as well as in mitophagy. Functions upstream of the ATG12-ATG5-ATG16L1 complex and LC3, and downstream of the ULK1 and PI3-kinase complexes. Involved in xenophagy of Staphylococcus aureus. Invading S.aureus cells become entrapped in autophagosome-like WIPI1 positive vesicles targeted for lysosomal degradation. Plays also a distinct role in controlling the transcription of melanogenic enzymes and melanosome maturation, a process that is distinct from starvation-induced autophagy. May also regulate the trafficking of proteins involved in the mannose-6-phosphate receptor (MPR) recycling pathway. |
| Molecular Weight | Theoretical: 49 kDa. Actual: 48 kDa. |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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