Anti-ATG12 Antibody (3P604) is a Rabbit antibody targeting ATG12. Anti-ATG12 Antibody (3P604) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,IP,FCM.

IgG

3P604

Human

1. Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ATG12 Monoclonal Antibody, Unconjugated (TMAB-02908) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ATG12 Monoclonal Antibody, Unconjugated (TMAB-02908) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
3. 25 μg total protein per lane of various lysates (see on figure) probed with ATG12 monoclonal antibody, unconjugated (TMAB-02908) at 1: 2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
4. The PC-3 (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-ATG12 antibody (TMAB-02908, 1:100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.

WB: 1:500-2000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; ICC/IF: 1:50-200; IF: 1:50-200; IP: 1:20-50; FCM: 1:50-100

Monoclonal

A synthesized peptide: human ATG12

Human

ATG12

Ubiquitin-like protein ATG12

Ubiquitin-like protein required for autophagy. Conjugated to ATG3 and ATG5.