Anti-ATG10 Antibody (6E579) is a Rabbit antibody targeting ATG10. Anti-ATG10 Antibody (6E579) can be used in FCM, IP, WB.

IgG

6E579

Human

1. Western blot analysis of HeLa cell lysate. Using ATG10 (TMAB-02905) monoclonal antibody at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. The K562 (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-ATG10 antibody (TMAB-02905, 1: 100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.

FCM=1:50-100; IP=1:20-50; WB=1:1000-2000

Monoclonal

A synthesized peptide: human ATG10

Human

ATG10

Ubiquitin-like-conjugating enzyme ATG10

E2-like enzyme involved in autophagy. Acts as an E2-like enzyme that catalyzes the conjugation of ATG12 to ATG5. ATG12 conjugation to ATG5 is required for autophagy. Likely serves as an ATG5-recognition molecule. Not involved in ATG12 conjugation to ATG3 (By similarity).