Anti-ATF5 Antibody (1S976) is a Rabbit antibody targeting ATF5. Anti-ATF5 Antibody (1S976) can be used in FCM, IP, WB.

IgG

1S976

Human,Mouse,Rat

1. Western blot analysis of (1) Jurkat cell lysate; (2) 3T3 cell lysate; (3) C6 cell lysate. Using ATF5 (TMAB-02902) monoclonal antibody at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. The A431 (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-ATF5 antibody (TMAB-02902, 1: 100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.

FCM=1:50-100; IP=1:20-50; WB=1:500-2000

Monoclonal

A synthesized peptide: human ATF5

Human

ATF5

Cyclic AMP-dependent transcription factor ATF-5

ATF5 or Activating transcription factor 5, binds to cAMP inducible promoters and is involved in gene transcription. This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. ATF5 plays a role in inhibition of nerve growth factor induced neuronal outgrowth and regulation of neurogenesis.