Anti-ATF3 Antibody (8F492) is a Rabbit antibody targeting ATF3. Anti-ATF3 Antibody (8F492) can be used in WB,IHC-P,IHC-Fr,IF.

IgG

8F492

Human,Mouse

1. RAW264.7 (M) cells were treated with or without LPS (1ug/ml) for 2 h, 25 μg total protein per lane of cell lysates (see on figure) probed with ATF3 monoclonal antibody, unconjugated (TMAB-02899) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ATF3 Monoclonal Antibody, Unconjugated (TMAB-02899) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.

WB: 1:500-1000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; IF: 1:50-200

Monoclonal

KLH conjugated synthetic peptide: human ATF

Human

ATF3

Cyclic AMP-dependent transcription factor ATF-3

This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Represses transcription from promoters with ATF sites. It may repress transcription by stabilizing the binding of inhibitory cofactors at the promoter. Isoform 2 activates transcription presumably by sequestering inhibitory cofactors away from the promoters.