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Anti-ATF2 Antibody (4L818)
😃Good
Catalog No. TMAC-00306
Alias TREB7, HB16, CRE-BP1, CREB-2, CREB2, activating transcription factor 2
Anti-ATF2 Antibody (4L818) is a Rabbit antibody targeting ATF2. Anti-ATF2 Antibody (4L818) can be used in ICC/IF,IHC,IP,WB.
Anti-ATF2 Antibody (4L818)
Anti-ATF2 Antibody (4L818)
😃Good
Catalog No. TMAC-00306Alias TREB7, HB16, CRE-BP1, CREB-2, CREB2, activating transcription factor 2
Anti-ATF2 Antibody (4L818) is a Rabbit antibody targeting ATF2. Anti-ATF2 Antibody (4L818) can be used in ICC/IF,IHC,IP,WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $298 | 7-10 days | 7-10 days | |
| 100 μL | $498 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-ATF2 Antibody (4L818) is a Rabbit antibody targeting ATF2. Anti-ATF2 Antibody (4L818) can be used in ICC/IF,IHC,IP,WB. |
| Synonyms | TREB7, HB16, CRE-BP1, CREB-2, CREB2, activating transcription factor 2 |
| Ig Type | IgG |
| Clone | 4L818 |
| Reactivity | Human |
| Verified Activity | 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-ATF2 antibody. Counter stained with hematoxylin. 2. ICC staining ATF2 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 3. ICC staining ATF2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. ICC staining ATF2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. ICC staining ATF2 in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.      |
| Application | |
| Recommended Dose | WB: 1:1000; IHC: 1:50-200; ICC/IF: 1:50-200 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Eukaryotic gene transcription is regulated by sequence-specific transcription factors which bind modular cis-acting promotor and enhancer elements. The ATF/CREB transcription factor family binds the palindromic cAMP response element (CRE) octanucleotide TGACGTCA. The ATF/CREB family includes CREB-1, CREB-2 (also designated ATF-4), ATF-1, ATF-2 and ATF-3. This family of proteins contain highly divergent N-terminal domains, but share a C-terminal leucine zipper for dimerization and DNA binding. ATF-2 forms homodimers and heterodimers with c-Jun to initiate CRE-dependent transcription. Phosphorylation of ATF-2 at Thr 69 and Thr 71 by stress-activated kinases is necessary for transcriptional activation. Myc also induces phosphorylation of ATF-2 at Thr 69 and Thr 71 to prolong the half-life of ATF-2. ATF-2 also functions as a histone acetyltransferase (HAT) by specifically acetylating histones H2B and H4 in vitro. The gene encoding human ATF-2 maps to chromosome 2q31.1. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 54 kDa. |
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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