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Anti-ATF1 Antibody (7A32) (Synonyms: Cyclic AMP-dependent transcription factor ATF-1, cAMP-dependent transcript...)

Catalog No. TMAC-00298 Copy Product Info
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Anti-ATF1 Antibody (7A32) is a Rabbit antibody targeting ATF1. Anti-ATF1 Antibody (7A32) can be used in FCM,ICC/IF,IHC,WB.

Anti-ATF1 Antibody (7A32)

Copy Product Info
🥰Excellent
Catalog No. TMAC-00298
Synonyms Cyclic AMP-dependent transcription factor ATF-1, cAMP-dependent transcription factor ATF-1, Activating transcription factor 1

Anti-ATF1 Antibody (7A32) is a Rabbit antibody targeting ATF1. Anti-ATF1 Antibody (7A32) can be used in FCM,ICC/IF,IHC,WB.

Anti-ATF1 Antibody
(7A32)
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2977-10 days7-10 days
100 μL$4987-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-ATF1 Antibody (7A32) is a Rabbit antibody targeting ATF1. Anti-ATF1 Antibody (7A32) can be used in FCM,ICC/IF,IHC,WB.
SynonymsCyclic AMP-dependent transcription factor ATF-1, cAMP-dependent transcription factor ATF-1, Activating transcription factor 1
Ig Type
IgG
Clone
7A32
Reactivity
Human
Verified Activity
1. Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-ATF1 antibody. Counter stained with hematoxylin.
2. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-ATF1 antibody. Counter stained with hematoxylin.
3. ICC staining ATF1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
4. ICC staining ATF1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
5. Flow cytometric analysis of Hela cells with ATF1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
verifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivity
Application
Recommended Dose
WB: 1;500-1000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionRecombinant Antibody
PurificationProA affinity purified
AppearanceLiquid
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Research BackgroundEukaryotic gene transcription is regulated by sequence-specific transcription factors that bind modular cis acting promoter and enhancer elements. The ATF/ CREB transcription factor family binds the palindromic cAMP response element (CRE) octanucleotide TGACGTCA. The ATF/CREB family includes CREB-1, CREB-2 (also designated ATF-4), ATF-1, ATF-2 and ATF-3. This family of proteins contain highly divergent N-terminal domains, but share a C-terminal leucine zipper for dimerization and DNA binding. ATF-1 is shown to play a key role in the induction of NOX1. ATF-1 binds the cAMP response element (CRE) and mediates PKA-induced stimulation of CRE-reporter genes. ATF-2 forms homodimers and heterodimers with c-Jun to initiate CRE-dependent transcription. Phosphorylation of ATF-2 at Thr 69 and Thr 71 by stress-activated kinases is necessary for transcriptional activation. Myc also induces phosphorylation of ATF-2 at Thr 69 and Thr 71 to prolong the half-life of ATF-2. ATF-2 functions as a histone acetyltransferase (HAT) and acetylates Histones H2B and H4 specifically in vitro.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 29 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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