Anti-ATF1 Antibody (4G420) is a Rabbit antibody targeting ATF1. Anti-ATF1 Antibody (4G420) can be used in WB,IHC-P,IHC-Fr,IF.

IgG

4G420

Human,Rat,Mouse

1. Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: 1: HeLa, 2: HCT-116, 3: K562, 4: HepG2
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 29 kDa
Observed MW: 35 kDa
2. Tissue: Rat liver
Section type: Formalin-fixed & Paraffin_x0002_ embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:100
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for TMAB-02896
3. Tissue: Human spleen
Section type: Formalin-fixed & Paraffin_x0002_ embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for TMAB-02896
4. Tissue: Mouse liver
Section type: Formalin-fixed & Paraffin_x0002_ embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:100
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for TMAB-02896

WB: 1:1000-5000; IHC-P: 1:100-1000; IHC-Fr: 1:100-1000; IF: 1:100-1000

Monoclonal

KLH conjugated synthetic peptide: human ATF1

Human

ATF1

Cyclic AMP-dependent transcription factor ATF-1

This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Binds to the Tax-responsive element (TRE) of HTLV-I. Mediates PKA-induced stimulation of CRE-reporter genes. Represses the expression of FTH1 and other antioxidant detoxification genes. Triggers cell proliferation and transformation.