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Anti-ATF-6 Polyclonal Antibody 2
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Catalog No. TMAB-00161
Alias Processed cyclic AMP-dependent transcription factor ATF-6 alpha, FLJ21663, ESTM49, DKFZp686P2194, Cyclic AMP dependent transcription factor ATF 6 alpha, cAMP-dependent transcription factor ATF-6 alpha, ATF6A, ATF6 alpha, ATF6, ATF 6, Activating transcription factor 6 alpha, Activating transcription factor 6
Anti-ATF-6 Polyclonal Antibody 2 is a Rabbit antibody targeting ATF-6. Anti-ATF-6 Polyclonal Antibody 2 can be used in IF,IHC-Fr,IHC-P,WB.
Anti-ATF-6 Polyclonal Antibody 2
Anti-ATF-6 Polyclonal Antibody 2
😃Good
Catalog No. TMAB-00161Alias Processed cyclic AMP-dependent transcription factor ATF-6 alpha, FLJ21663, ESTM49, DKFZp686P2194, Cyclic AMP dependent transcription factor ATF 6 alpha, cAMP-dependent transcription factor ATF-6 alpha, ATF6A, ATF6 alpha, ATF6, ATF 6, Activating transcription factor 6 alpha, Activating transcription factor 6
Anti-ATF-6 Polyclonal Antibody 2 is a Rabbit antibody targeting ATF-6. Anti-ATF-6 Polyclonal Antibody 2 can be used in IF,IHC-Fr,IHC-P,WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $220 | 7-10 days | 7-10 days | |
| 100 μL | $372 | 7-10 days | 7-10 days | |
| 200 μL | $529 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-ATF-6 Polyclonal Antibody 2 is a Rabbit antibody targeting ATF-6. Anti-ATF-6 Polyclonal Antibody 2 can be used in IF,IHC-Fr,IHC-P,WB. |
| Synonyms | Processed cyclic AMP-dependent transcription factor ATF-6 alpha, FLJ21663, ESTM49, DKFZp686P2194, Cyclic AMP dependent transcription factor ATF 6 alpha, cAMP-dependent transcription factor ATF-6 alpha, ATF6A, ATF6 alpha, ATF6, ATF 6, Activating transcription factor 6 alpha, Activating transcription factor 6 |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Dog,Pig,Cow,Horse,Rabbit,Sheep) |
| Verified Activity | 1. Sample: Lane 1: Human HUVEC cell Lysates Lane 2: Human Raji cell Lysates Lane 3: Human Jurkat cell Lysates Lane 4: Human Hela cell Lysates Primary: Anti-ATF6 (TMAB-00161) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 75 kDa Observed band size: 110 kDa 2. Sample: K562 (Human) Cell Lysate at 30 μg Primary: Anti-ATF6 (TMAB-00161) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 75 kDa Observed band size: 75 kDa 3. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ATF6) Polyclonal Antibody, Unconjugated (TMAB-00161) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ATF6) Polyclonal Antibody, Unconjugated (TMAB-00161) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.     |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Endoplasmic reticulum membrane; Single-pass type II membrane protein. Processed cyclic AMP-dependent transcription factor ATF-6 alpha: Nucleus. Note=Under ER stress the cleaved N-terminal cytoplasmic domain translocates into the nucleus. |
| Tissue Specificity | Ubiquitous. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1 mg/mL |
| Research Background | ATF6 is a transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. It binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. ATF6 could also be involved in activation of transcription by the serum response factor. ATF6 exists as a homodimer and heterodimer with ATF6 beta. The dimer interacts with the nuclear transcription factor Y (NF-Y) trimer through direct binding to NF-Y subunit C (NF-YC). It also interacts with the transcription factors GTF2I, YY1 and SRF. Under ER stress the cleaved N-terminal cytoplasmic domain translocates into the nucleus. The basic domain of ATF6 functions as a nuclear localization signal and the basic leucine zipper domain is sufficient for association with the NF-Y trimer and binding to ERSE. During the unfolded protein response an approximately 50 kDa fragment containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage seems to be performed sequentially by site 1 and site 2 proteases. ATF6 is N glycosylated, phosphorylated in vitro by MAPK14/P38MAPK and belongs to the bZIP family. |
| Immunogen | KLH conjugated synthetic peptide: human ATF6 |
| Antigen Species | Human |
| Gene Name | ATF6 |
| Gene ID | |
| Protein Name | Cyclic AMP-dependent transcription factor ATF-6 alpha |
| Uniprot ID | |
| Biology Area | ChIP antibodies,Leucine Zipper |
| Function | Transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. Binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor. |
| Molecular Weight | Theoretical: 75 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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