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Anti-ASNS Polyclonal Antibody

Catalog No. TMAB-02874 Copy Product Info
🥰Excellent
Anti-ASNS Polyclonal Antibody is a Rabbit antibody targeting ASNS. Anti-ASNS Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-ASNS Polyclonal Antibody

Copy Product Info
🥰Excellent
Catalog No. TMAB-02874

Anti-ASNS Polyclonal Antibody is a Rabbit antibody targeting ASNS. Anti-ASNS Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-ASNS Polyclonal Antibody
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2237-10 days7-10 days
100 μL$3717-10 days7-10 days
200 μL$5297-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-ASNS Polyclonal Antibody is a Rabbit antibody targeting ASNS. Anti-ASNS Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human,Mouse,Rat (predicted:Dog,Pig,Cow,Horse,Rabbit)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ASNS) Polyclonal Antibody, Unconjugated (TMAB-02874) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Asparagine synthetase) Polyclonal Antibody, Unconjugated (TMAB-02874) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ASNS) Polyclonal Antibody, Unconjugated (TMAB-02874) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ASNS) Polyclonal Antibody, Unconjugated (TMAB-02874) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded (mouse stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ASNS) Polyclonal Antibody, Unconjugated (TMAB-02874) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ASNS) Polyclonal Antibody, Unconjugated (TMAB-02874) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded (Human stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ASNS) Polyclonal Antibody, Unconjugated (TMAB-02874) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
8. Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ASNS) Polyclonal Antibody, Unconjugated (TMAB-02874) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
9. Sample:
Lane 1: Mouse Spleen tissue lysates
Lane 2: Mouse Cerebrum tissue lysates
Lane 3: Mouse Cerebellum tissue lysates
Lane 4: Rat Cerebrum tissue lysates
Lane 5: Rat Cerebellum tissue lysates
Lane 6: Human HeLa cell lysates
Lane 7: Human Jurkat cell lysates
Lane 8: Human SH-SY5Y cell lysates
Lane 9: Human U87MG cell lysates
Primary: Anti-ASNS (TMAB-02874) at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 64 kDa
Observed band size: 60 kDa
Application
Recommended Dose
WB: 1:1000-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundThe protein encoded by this gene is involved in the synthesis of asparagine. This gene complements a mutation in the temperature-sensitive hamster mutant ts11, which blocks progression through the G1 phase of the cell cycle at nonpermissive temperature. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human ASNS
Antigen Species
Human
Gene Name
ASNS
Gene ID
Protein Name
Asparagine synthetase [glutamine-hydrolyzing]
Uniprot ID
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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