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Anti-ASK1 Antibody (3O802)
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Catalog No. TMAC-00285
Alias Mitogen-activated protein kinase kinase kinase 5, MEKK5, MEKK 5, MEK kinase 5, MAPKKK5, MAPK/ERK kinase kinase 5, MAP3K5, M3K5, ASK-1, ASK1, Apoptosis signal-regulating kinase 1
Anti-ASK1 Antibody (3O802) is a Rabbit antibody targeting ASK1. Anti-ASK1 Antibody (3O802) can be used in WB,ICC/IF,IHC,FCM.
Anti-ASK1 Antibody (3O802)
Anti-ASK1 Antibody (3O802)
😃Good
Catalog No. TMAC-00285Alias Mitogen-activated protein kinase kinase kinase 5, MEKK5, MEKK 5, MEK kinase 5, MAPKKK5, MAPK/ERK kinase kinase 5, MAP3K5, M3K5, ASK-1, ASK1, Apoptosis signal-regulating kinase 1
Anti-ASK1 Antibody (3O802) is a Rabbit antibody targeting ASK1. Anti-ASK1 Antibody (3O802) can be used in WB,ICC/IF,IHC,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $296 | 7-10 days | |
| 100 μL | $497 | 7-10 days |
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Chemical Properties
| Description | Anti-ASK1 Antibody (3O802) is a Rabbit antibody targeting ASK1. Anti-ASK1 Antibody (3O802) can be used in WB,ICC/IF,IHC,FCM. |
| Synonyms | Mitogen-activated protein kinase kinase kinase 5, MEKK5, MEKK 5, MEK kinase 5, MAPKKK5, MAPK/ERK kinase kinase 5, MAP3K5, M3K5, ASK-1, ASK1, Apoptosis signal-regulating kinase 1 |
| Ig Type | IgG |
| Reactivity | Human,Mouse |
| Verified Activity | 1. Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-ASK1 antibody. Counter stained with hematoxylin. 2. Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-ASK1 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-ASK1 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-ASK1 antibody. Counter stained with hematoxylin. 5. ICC staining ASK1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining ASK1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining ASK1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. Flow cytometric analysis of Hela cells with ASK1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:1000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Mitogen-activated protein (MAP) kinase cascades are activated by various extracellular stimuli including growth factors. The MEK kinases (also designated MAP kinase kinase kinases, MKKKs, MAP3Ks or MEKKs) phosphorylate and thereby activate the MEKs (also called MAP kinase kinases or MKKs), including ERK, JNK and p38. These activated MEKs in turn phosphorylate and activate the MAP kinases. The MEK kinases include Raf-1, Raf-B, Mos, MEK kinase-1, MEK kinase-2, MEK kinase-3, MEK kinase-4, ASK 1 (MEK kinase-5) and MAP3K6 (MEK kinase-6). MEK kinase-1 has been shown to phosphorylate MEK-1 via a Raf-independent pathway. Evidence suggests that MEK-3 is preferentially activated by MEK kinase-3 and that MEK-4 is activated by both MEK kinase-2 and MEK kinase-3. MEK kinase-4 has been shown to specifically activate the JNK pathway. ASK1 activates both MEK-4 and MEK-3/MEK-6 pathways. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 155 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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