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Anti-ASH2L Antibody (4J278)
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Catalog No. TMAC-00282
Alias Set1/Ash2 histone methyltransferase complex subunit ASH2, ASH2-like protein, ASH2L1, ASH2L
Anti-ASH2L Antibody (4J278) is a Rabbit antibody targeting ASH2L. Anti-ASH2L Antibody (4J278) can be used in WB,ICC/IF,IHC.
Anti-ASH2L Antibody (4J278)
Anti-ASH2L Antibody (4J278)
😃Good
Catalog No. TMAC-00282Alias Set1/Ash2 histone methyltransferase complex subunit ASH2, ASH2-like protein, ASH2L1, ASH2L
Anti-ASH2L Antibody (4J278) is a Rabbit antibody targeting ASH2L. Anti-ASH2L Antibody (4J278) can be used in WB,ICC/IF,IHC.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $297 | 7-10 days | |
| 100 μL | $497 | 7-10 days |
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| Description | Anti-ASH2L Antibody (4J278) is a Rabbit antibody targeting ASH2L. Anti-ASH2L Antibody (4J278) can be used in WB,ICC/IF,IHC. |
| Synonyms | Set1/Ash2 histone methyltransferase complex subunit ASH2, ASH2-like protein, ASH2L1, ASH2L |
| Ig Type | IgG |
| Clone | 4J278 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of ASH2L on different lysates using anti-ASH2L antibody at 1/1,000 dilution. Positive control: Lane 1: PC12, Lane 2: 293T. 2. Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-ASH2L antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-ASH2L antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse ovary tissue using anti-ASH2L antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-ASH2L antibody. Counter stained with hematoxylin. 6. ICC staining ASH2L in PC12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining ASH2L in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. ICC staining ASH2L in CRC cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
| Application | |
| Recommended Dose | WB: 1:1000-5000; IHC: 1:200-500; ICC/IF: 1:200-500 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | The human ASH2L gene encodes a 628 amino acid protein known as ASH2L1, or isoform 1, which contains a nuclear localization signal and PHD finger motif, suggesting that the gene product functions as a transcription regulator. Alternative splicing results in a shorter isoform 2, designated ASH2L2, which is missing the first 94 amino acid residues found in ASH2L1. Human ASH2L proteins are 60% homologous to Drosophila ash2, which positively regulates expression of certain genes in early development, and contain similar, but not identical, domains, including a zinc finger motif. ASH2L is highly expressed in fetal liver, testis and leukemia cell lines with erythroid and megakaryocytic potential, such as K562, Hel and Dami. Differentiation inducers (e.g. phorbol ester and hemin) cause different expression patterns in these cells lines, suggesting that ASH2L plays a role in hematopoiesis and is associated with particular types of leukemia. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 69 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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