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Anti-ASF1B Antibody (3P394)
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Catalog No. TMAY-02725
Alias CIA-II, anti-silencing function 1B histone chaperone
Anti-ASF1B Antibody (3P394) is a Mouse antibody targeting ASF1B. Anti-ASF1B Antibody (3P394) can be used in ICC/IF.
Anti-ASF1B Antibody (3P394)
Anti-ASF1B Antibody (3P394)
😃Good
Catalog No. TMAY-02725Alias CIA-II, anti-silencing function 1B histone chaperone
Anti-ASF1B Antibody (3P394) is a Mouse antibody targeting ASF1B. Anti-ASF1B Antibody (3P394) can be used in ICC/IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $199 | 7-10 days |
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Antigen Details
Chemical Properties
| Description | Anti-ASF1B Antibody (3P394) is a Mouse antibody targeting ASF1B. Anti-ASF1B Antibody (3P394) can be used in ICC/IF. |
| Alias | CIA-II, anti-silencing function 1B histone chaperone |
| Ig Type | Monoclonal Mouse IgG2b |
| Clone | 3P394 |
| Reactivity | Human |
| Verified Activity | Immunofluorescence staining of Human ASF1B in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human ASF1B monoclonal antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green). Positive staining was localized to nucleus. |
| Application | ICC/IF |
| Recommended Dose | ICC-IF: 1:20-1:100 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human ASF1B (rh ASF1B; TMPY-03341; Q9NVP2; Met1-Ile202). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | The histone chaperone anti-silencing factor 1a (ASF1a) interacts with MDC1 and is recruited to sites of DSBs to facilitate the interaction of phospho-ATM with MDC1 and phosphorylation of MDC1, which are required for the recruitment of RNF8/RNF168 histone ubiquitin ligases. Thus, ASF1a deficiency reduces histone ubiquitination at DSBs, decreasing the recruitment of 53BP1, and decreases NHEJ, rendering cells more sensitive to DSBs. This role of ASF1a in DSB repair cannot be provided by the closely related ASF1b and does not require its histone chaperone activity. Homozygous deletion of ASF1A is seen in 10%-15% of certain cancers, suggesting that loss of NHEJ may be selected in some malignancies and that the deletion can be used as a molecular biomarker for cancers susceptible to radiotherapy or to DSB-inducing chemotherapy. Anti-silencing function 1 (ASF1) is a histone H3-H4 chaperone involved in DNA replication and repair, and transcriptional regulation. Here, we identify ASF1B, the mammalian paralog to ASF1, as a proliferation-inducing histone chaperone in human β-cells. Overexpression of ASF1B led to distinct transcriptional signatures consistent with increased cellular proliferation and reduced cellular death. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Human ASF1B protein (TMPY-03341) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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