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Anti-ASAP3 Polyclonal Antibody
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Catalog No. TMAB-02856
Anti-ASAP3 Polyclonal Antibody is a Rabbit antibody targeting ASAP3. Anti-ASAP3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-ASAP3 Polyclonal Antibody
Anti-ASAP3 Polyclonal Antibody
Copy Product Info😃Good
Catalog No. TMAB-02856
Anti-ASAP3 Polyclonal Antibody is a Rabbit antibody targeting ASAP3. Anti-ASAP3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $223 | 7-10 days | 7-10 days | |
| 100 μL | $371 | 7-10 days | 7-10 days | |
| 200 μL | $529 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Antigen Details
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| Description | Anti-ASAP3 Polyclonal Antibody is a Rabbit antibody targeting ASAP3. Anti-ASAP3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Mouse,Rat (predicted:Human,Dog,Pig,Cow,Horse,Sheep) |
| Verified Activity | 1. Sample: Liver (Mouse) Lysate at 40 μg Primary: Anti-ASAP3 (TMAB-02856) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 99 kD Observed band size: 100 kD 2. Paraformaldehyde-fixed, paraffin embedded (mouse stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ASAP3) Polyclonal Antibody, Unconjugated (TMAB-02856) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ASAP3) Polyclonal Antibody, Unconjugated (TMAB-02856) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 4. Sample: Lane 1: Mouse Cerebrum tissue lysates Lane 2: Mouse Lung tissue lysates Primary: Anti-ASAP3 (TMAB-02856) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 99 kDa Observed band size: 105 kDa |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Tissue Specificity | Highly expressed in primary hepatocarcinoma. Detected in lung, liver and blood leukocytes. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | ASAP3 is a member of a subfamily of ADP-ribosylation factor(Arf) GTPase-activating proteins that contain additional ankyrin repeat and pleckstrin homology domains. The Arf GAP domain of this protein catalyzes the hydrolysis of GTP bound to Arf proteins. The encoded protein promotes cell differentiation and migration and has been implicated in cancer cell invasion. |
| Immunogen | KLH conjugated synthetic peptide: human ASAP3 |
| Antigen Species | Human |
| Gene Name | ASAP3 |
| Gene ID | |
| Protein Name | Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 3 |
| Uniprot ID | |
| Function | Promotes cell proliferation. |
| Molecular Weight | Theoretical: 99 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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