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Anti-Arp3/ACTR3 Antibody (1Q436)
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Catalog No. TMAC-00273
Alias ARP3 actin-related protein 3 homolog (yeast), ARP3
Anti-Arp3/ACTR3 Antibody (1Q436) is a Rabbit antibody targeting Arp3/ACTR3. Anti-Arp3/ACTR3 Antibody (1Q436) can be used in FCM,ICC,IF,IHC,WB.
Anti-Arp3/ACTR3 Antibody (1Q436)
Anti-Arp3/ACTR3 Antibody (1Q436)
😃Good
Catalog No. TMAC-00273Alias ARP3 actin-related protein 3 homolog (yeast), ARP3
Anti-Arp3/ACTR3 Antibody (1Q436) is a Rabbit antibody targeting Arp3/ACTR3. Anti-Arp3/ACTR3 Antibody (1Q436) can be used in FCM,ICC,IF,IHC,WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $296 | 7-10 days | |
| 100 μL | $497 | 7-10 days |
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| Description | Anti-Arp3/ACTR3 Antibody (1Q436) is a Rabbit antibody targeting Arp3/ACTR3. Anti-Arp3/ACTR3 Antibody (1Q436) can be used in FCM,ICC,IF,IHC,WB. |
| Synonyms | ARP3 actin-related protein 3 homolog (yeast), ARP3 |
| Ig Type | IgG |
| Clone | 1Q436 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of Arp3 on different lysates using anti-Arp3 antibody at 1/500 dilution.Positive control:Lane 1: A431, Lane 2: Mouse placenta, Lane 3: Mouse thymus. 2. Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-Arp3 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Arp3 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-Arp3 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Arp3 antibody. Counter stained with hematoxylin. 6. ICC staining Arp3 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining Arp3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. ICC staining Arp3 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 9. Flow cytometric analysis of HL-60 cells with Arp3 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:500-1000; IHC: 1:50-200; ICC: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Actin polymerization is required for a variety of cell functions, including chemotaxis, cell migration, cell adhesion, and platelet activation. Cells trigger actin polymerization through either the de novo nucleation of filaments from monomeric actin, the severing of existing filaments to create uncapped barbed ends, or the uncapping existing barbed ends. The nucleation of actin is a rate-limiting and unfavorable reaction in actin polymerization and therefore requires the involvement of the Arp2/3 complex, which helps create new filaments and promotes the end-to-side cross-linking of actin filaments into the branching meshwork. The Arp2/3 complex consists of the actin-related proteins Arp2 and Arp3, and various other accessory proteins. The Arp2/3 complex promotes actin nucleation by binding the pointed end of actin filaments, or by associating with the side of an existing filament, and nucleates growth in the barbed direction. In addition, the Arp2/3 complex also mediates actin cytoskeletal outgrowths that are regulated by the Rho family of small GTPases. In response to GTP-binding Cdc42, the Arp2/3 complex binds the Cdc42 substrates, namely the WASP proteins, and initiates the formation of lamellipodia and filopodia. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: C-terminal human Arp3 |
| Antigen Species | human |
| Uniprot ID |
| Molecular Weight | Theoretical: 47 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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