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Anti-Aromatase/CYP19A1 Polyclonal Antibody
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Catalog No. TMAB-00148
Alias Estrogen synthase, Cytochrome P-450AROM, Cytochrome P450 19A1, CYPXIX, CYP19A1, CYP19, CYAR, Aromatase, ARO1
Anti-Aromatase/CYP19A1 Polyclonal Antibody is a Rabbit antibody targeting Aromatase/CYP19A1. Anti-Aromatase/CYP19A1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-Aromatase/CYP19A1 Polyclonal Antibody
Anti-Aromatase/CYP19A1 Polyclonal Antibody
😃Good
Catalog No. TMAB-00148Alias Estrogen synthase, Cytochrome P-450AROM, Cytochrome P450 19A1, CYPXIX, CYP19A1, CYP19, CYAR, Aromatase, ARO1
Anti-Aromatase/CYP19A1 Polyclonal Antibody is a Rabbit antibody targeting Aromatase/CYP19A1. Anti-Aromatase/CYP19A1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-Aromatase/CYP19A1 Polyclonal Antibody is a Rabbit antibody targeting Aromatase/CYP19A1. Anti-Aromatase/CYP19A1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Alias | Estrogen synthase, Cytochrome P-450AROM, Cytochrome P450 19A1, CYPXIX, CYP19A1, CYP19, CYAR, Aromatase, ARO1 |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Dog,Pig,Cow,Horse,Rabbit,Sheep) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Aromatase) Polyclonal Antibody, Unconjugated (TMAB-00148) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. 2. Sample: K562 (human) cell Lysate at 40 μg Primary: Anti-Aromatase (TMAB-00148) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kDa Observed band size: 55 kDa 3. Paraformaldehyde-fixed, paraffin embedded (rat ovary tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Aromatase) Polyclonal Antibody, Unconjugated (TMAB-00148) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Membrane; Peripheral membrane protein. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | Aromatase is a key enzyme in steroidogenesis and plays an important role in sexual differentiation, oestrogen biosynthesis, fertility and carcinogenesis. It is highly conserved amongst mammals, and is highly expressed in placental tissue. Many environmental chemicals may influence aromatase activity and thereby disrupt endocrine function. |
| Immunogen | KLH conjugated synthetic peptide: human Aromatase |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Cytochromes,Drug metabolism,Lipases,Cytochromes,Cancer,Hormones,Drug metabolism |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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