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Anti-Aromatase/CYP19A1 Polyclonal Antibody

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Catalog No. TMAB-00148

Anti-Aromatase/CYP19A1 Polyclonal Antibody is a Rabbit antibody targeting Aromatase/CYP19A1. Anti-Aromatase/CYP19A1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-Aromatase/CYP19A1 Polyclonal Antibody

Anti-Aromatase/CYP19A1 Polyclonal Antibody

😃Good
Catalog No. TMAB-00148
Anti-Aromatase/CYP19A1 Polyclonal Antibody is a Rabbit antibody targeting Aromatase/CYP19A1. Anti-Aromatase/CYP19A1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$220 7-10 days
100 μL$372 7-10 days
200 μL$529 7-10 days
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Product Introduction

Bioactivity
Description
Anti-Aromatase/CYP19A1 Polyclonal Antibody is a Rabbit antibody targeting Aromatase/CYP19A1. Anti-Aromatase/CYP19A1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human,Mouse,Rat (predicted:Dog,Pig,Cow,Horse,Rabbit,Sheep)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Aromatase) Polyclonal Antibody, Unconjugated (TMAB-00148) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
2. Sample: K562 (human) cell Lysate at 40 μg
Primary: Anti-Aromatase (TMAB-00148) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kDa
Observed band size: 55 kDa
3. Paraformaldehyde-fixed, paraffin embedded (rat ovary tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Aromatase) Polyclonal Antibody, Unconjugated (TMAB-00148) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
Application
WB,IHC-P,IHC-Fr,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationMembrane; Peripheral membrane protein.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundAromatase is a key enzyme in steroidogenesis and plays an important role in sexual differentiation, oestrogen biosynthesis, fertility and carcinogenesis. It is highly conserved amongst mammals, and is highly expressed in placental tissue. Many environmental chemicals may influence aromatase activity and thereby disrupt endocrine function.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human Aromatase
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Cytochromes,Drug metabolism,Lipases,Cytochromes,Cancer,Hormones,Drug metabolism
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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