Anti-Aromatase/CYP19A1 Polyclonal Antibody is a Rabbit antibody targeting Aromatase/CYP19A1. Anti-Aromatase/CYP19A1 Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.

IgG

Human,Mouse,Rat (predicted:Dog,Pig,Cow,Horse,Rabbit,Sheep)

1. Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Aromatase) Polyclonal Antibody, Unconjugated (TMAB-00148) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
2. Sample: K562 (human) cell Lysate at 40 μg
Primary: Anti-Aromatase (TMAB-00148) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kDa
Observed band size: 55 kDa
3. Paraformaldehyde-fixed, paraffin embedded (rat ovary tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Aromatase) Polyclonal Antibody, Unconjugated (TMAB-00148) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human Aromatase

Human

CYP19A1

Aromatase

Cytochromes,Drug metabolism,Lipases,Cytochromes,Cancer,Hormones,Drug metabolism

Catalyzes the formation of aromatic C18 estrogens from C19 androgens.