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Anti-Argininosuccinate synthase 1 Antibody (2S146)

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Catalog No. TMAB-02819

Anti-Argininosuccinate synthase 1 Antibody (2S146) is a Rabbit antibody targeting Argininosuccinate synthase 1. Anti-Argininosuccinate synthase 1 Antibody (2S146) can be used in WB,IHC-P,IHC-Fr,IF,IP,FCM.

Anti-Argininosuccinate synthase 1 Antibody (2S146)

Anti-Argininosuccinate synthase 1 Antibody (2S146)

Copy Product Info
😃Good
Catalog No. TMAB-02819
Anti-Argininosuccinate synthase 1 Antibody (2S146) is a Rabbit antibody targeting Argininosuccinate synthase 1. Anti-Argininosuccinate synthase 1 Antibody (2S146) can be used in WB,IHC-P,IHC-Fr,IF,IP,FCM.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
25 μL$1497-10 days7-10 days
50 μL$2627-10 days7-10 days
100 μL$4737-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-Argininosuccinate synthase 1 Antibody (2S146) is a Rabbit antibody targeting Argininosuccinate synthase 1. Anti-Argininosuccinate synthase 1 Antibody (2S146) can be used in WB,IHC-P,IHC-Fr,IF,IP,FCM.
Ig Type
IgG
Clone
2S146
Reactivity
Human,Mouse,Rat
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Argininosuccinate synthase 1 Monoclonal Antibody, Unconjugated (TMAB-02819) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Argininosuccinate synthase 1 Monoclonal Antibody, Unconjugated (TMAB-02819) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Argininosuccinate synthase 1 Monoclonal Antibody, Unconjugated (TMAB-02819) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Argininosuccinate synthase 1 Monoclonal Antibody, Unconjugated (TMAB-02819) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Argininosuccinate synthase 1 Monoclonal Antibody, Unconjugated (TMAB-02819) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Argininosuccinate synthase 1 Monoclonal Antibody, Unconjugated (TMAB-02819) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Human Ovarian Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Argininosuccinate synthase 1 Monoclonal Antibody, Unconjugated (TMAB-02819) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
8. 25 ug total protein per lane of various lysates (see on figure) probed with Argininosuccinate synthase 1 monoclonal antibody, unconjugated (TMAB-02819) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; IP: 1:20-50; FCM: 1:50-100
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionRecombinant Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation10mM phosphate buffered saline (pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
Research BackgroundOne of the enzymes of the urea cycle, the metabolic pathway transforming neurotoxic amonia produced by protein catabolism into inocuous urea in the liver of ureotelic animals. Catalyzes the formation of arginosuccinate from aspartate, citrulline and ATP and together with ASL it is responsible for the biosynthesis of arginine in most body tissues.
Antigen Details
Immunogen
A synthesized peptide: human Argininosuccinate synthase 1
Antigen Species
Human
Gene Name
ASS1
Gene ID
Protein Name
Argininosuccinate synthase
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 47 kDa. Actual: 47 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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