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Anti-Argininosuccinate synthase 1 Antibody (2S146) is a Rabbit antibody targeting Argininosuccinate synthase 1. Anti-Argininosuccinate synthase 1 Antibody (2S146) can be used in WB,IHC-P,IHC-Fr,IF,IP,FCM.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 25 μL | $149 | 7-10 days | 7-10 days | |
| 50 μL | $262 | 7-10 days | 7-10 days | |
| 100 μL | $473 | 7-10 days | 7-10 days |
| Description | Anti-Argininosuccinate synthase 1 Antibody (2S146) is a Rabbit antibody targeting Argininosuccinate synthase 1. Anti-Argininosuccinate synthase 1 Antibody (2S146) can be used in WB,IHC-P,IHC-Fr,IF,IP,FCM. |
| Ig Type | IgG |
| Clone | 2S146 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Argininosuccinate synthase 1 Monoclonal Antibody, Unconjugated (TMAB-02819) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Argininosuccinate synthase 1 Monoclonal Antibody, Unconjugated (TMAB-02819) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Argininosuccinate synthase 1 Monoclonal Antibody, Unconjugated (TMAB-02819) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Argininosuccinate synthase 1 Monoclonal Antibody, Unconjugated (TMAB-02819) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Argininosuccinate synthase 1 Monoclonal Antibody, Unconjugated (TMAB-02819) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Argininosuccinate synthase 1 Monoclonal Antibody, Unconjugated (TMAB-02819) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining. 7. Paraformaldehyde-fixed, paraffin embedded Human Ovarian Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Argininosuccinate synthase 1 Monoclonal Antibody, Unconjugated (TMAB-02819) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining. 8. 25 ug total protein per lane of various lysates (see on figure) probed with Argininosuccinate synthase 1 monoclonal antibody, unconjugated (TMAB-02819) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; IP: 1:20-50; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 10mM phosphate buffered saline (pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| Research Background | One of the enzymes of the urea cycle, the metabolic pathway transforming neurotoxic amonia produced by protein catabolism into inocuous urea in the liver of ureotelic animals. Catalyzes the formation of arginosuccinate from aspartate, citrulline and ATP and together with ASL it is responsible for the biosynthesis of arginine in most body tissues. |
| Immunogen | A synthesized peptide: human Argininosuccinate synthase 1 |
| Antigen Species | Human |
| Gene Name | ASS1 |
| Gene ID | |
| Protein Name | Argininosuccinate synthase |
| Uniprot ID |
| Molecular Weight | Theoretical: 47 kDa. Actual: 47 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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