Antibody Type: Rabbit Polyclonal

Application: FCM

Reactivity: Human (predicted:Mouse,Rat)

IgG

Human (predicted:Mouse,Rat)

1. Blank control (black line): A431. Primary Antibody (green line): Rabbit Anti-AQP3 antibody (TMAB-00136)
Dilution: 1 μg/Test;
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488
Dilution: 0.5 μg/Test.
Isotype control (orange line): Normal Rabbit IgG
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
2. Blank control: Hela.
Primary Antibody (green line): Rabbit Anti-AQP3 antibody (TMAB-00136)
Dilution: 3 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 3 μg/test.
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.

FCM

FCM: 2ug/Test

Polyclonal

KLH conjugated synthetic peptide: mouse AQP3

Mouse

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