Anti-Aquaporin-1/AQP1 Polyclonal Antibody is a Rabbit antibody targeting Aquaporin-1/AQP1. Anti-Aquaporin-1/AQP1 Polyclonal Antibody can be used in IF, IHC-Fr, IHC-P.

IgG

Human,Mouse,Rat (predicted:Dog,Pig,Cow,Horse,Sheep)

1. Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-AQP-1 Polyclonal Antibody, Unconjugated (TMAB-00135) 1:400, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (AQP1) Polyclonal Antibody, Unconjugated (TMAB-00135) at 1:200 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (AQP1) Polyclonal Antibody, Unconjugated (TMAB-00135) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (AQP1) Polyclonal Antibody, Unconjugated (TMAB-00135) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

IF=1:200-500; IHC-Fr=1:200-500; IHC-P=1:200-500

Polyclonal

KLH conjugated synthetic peptide: human AQP1

Human

AQP1

Aquaporin-1

Channels

Forms a water-specific channel that provides the plasmamembranes of red cells and kidney proximal tubules with highpermeability to water, thereby permitting water to move in thedirection of an osmotic gradient.