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Anti-Aquaporin-1/AQP1 Polyclonal Antibody
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Catalog No. TMAB-00135
Anti-Aquaporin-1/AQP1 Polyclonal Antibody is a Rabbit antibody targeting Aquaporin-1/AQP1. Anti-Aquaporin-1/AQP1 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Anti-Aquaporin-1/AQP1 Polyclonal Antibody
Anti-Aquaporin-1/AQP1 Polyclonal Antibody
😃Good
Catalog No. TMAB-00135
Anti-Aquaporin-1/AQP1 Polyclonal Antibody is a Rabbit antibody targeting Aquaporin-1/AQP1. Anti-Aquaporin-1/AQP1 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $222 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: IHC-P,IHC-Fr,IF Reactivity: Human,Mouse,Rat (predicted:Dog,Pig,Cow,Horse,Sheep) |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Dog,Pig,Cow,Horse,Sheep) |
| Verified Activity | 1. Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-AQP-1 Polyclonal Antibody, Unconjugated (TMAB-00135) 1:400, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 2. Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (AQP1) Polyclonal Antibody, Unconjugated (TMAB-00135) at 1:200 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (AQP1) Polyclonal Antibody, Unconjugated (TMAB-00135) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (AQP1) Polyclonal Antibody, Unconjugated (TMAB-00135) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
| Application | IHC-P,IHC-Fr,IF |
| Recommended Dose | IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Membrane; Multi-pass membrane protein. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | Aquaporin 1 is a 28kD integral membrane protein which was originally identified in red blood cells and renal proximal tubules. AQP1 is also expressed by the choroid plexus and various other tissues. It forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. |
| Immunogen | KLH conjugated synthetic peptide: human AQP1 |
| Antigen Species | Human |
| Gene ID | 358 |
| Uniprot ID | |
| Biology Area | Channels |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Sci Citations
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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