Anti-AQP2 Polyclonal Antibody is a Rabbit antibody targeting AQP2. Anti-AQP2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat

1. Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-AQP-2 Polyclonal Antibody, Unconjugated (TMAB-02793) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Sample:
Kidney (Mouse) Lysate at 40 μg
293T (Human) Cell Lysate at 30 μg
Primary: Anti-Aquaporin 2 (TMAB-02793) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 30 kD
Observed band size: 32 kD
3. Sample: Heart (Mouse) Lysate at 40 μg
Primary: Anti-Aquaporin 2 (TMAB-02793) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 30 kD
Observed band size: 35 kD

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human AQP2

Human

AQP2

Aquaporin-2

Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.