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Anti-AQP1 Antibody (2H736) is a Rabbit antibody targeting AQP1. Anti-AQP1 Antibody (2H736) can be used in WB,IF,IHC-P,IHC-Fr.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 25 μL | $149 | 7-10 days | 7-10 days | |
| 50 μL | $263 | 7-10 days | 7-10 days | |
| 100 μL | $473 | 7-10 days | 7-10 days |
| Description | Anti-AQP1 Antibody (2H736) is a Rabbit antibody targeting AQP1. Anti-AQP1 Antibody (2H736) can be used in WB,IF,IHC-P,IHC-Fr. |
| Ig Type | IgG |
| Clone | 2H736 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AQP1 Monoclonal Antibody, Unconjugated (TMAB-02791) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded Rat Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AQP1 Monoclonal Antibody, Unconjugated (TMAB-02791) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Human Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AQP1 Monoclonal Antibody, Unconjugated (TMAB-02791) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AQP1 Monoclonal Antibody, Unconjugated (TMAB-02791) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AQP1 Monoclonal Antibody, Unconjugated (TMAB-02791) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AQP1 Monoclonal Antibody, Unconjugated (TMAB-02791) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 7. Paraformaldehyde-fixed, paraffin embedded Mouse Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AQP1 Monoclonal Antibody, Unconjugated (TMAB-02791) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 8. Paraformaldehyde-fixed, paraffin embedded Rat Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AQP1 Monoclonal Antibody, Unconjugated (TMAB-02791) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 9. Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AQP1 Monoclonal Antibody, Unconjugated (TMAB-02791) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 10. Paraformaldehyde-fixed, paraffin embedded Rat Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AQP1 Monoclonal Antibody, Unconjugated (TMAB-02791) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 11. Paraformaldehyde-fixed, paraffin embedded Human Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AQP1 Monoclonal Antibody, Unconjugated (TMAB-02791) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 12. Paraformaldehyde-fixed, paraffin embedded Rat Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AQP1 Monoclonal Antibody, Unconjugated (TMAB-02791) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 13. Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AQP1 Monoclonal Antibody, Unconjugated (TMAB-02791) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 14. Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AQP1 Monoclonal Antibody, Unconjugated (TMAB-02791) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 15. Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AQP1 Monoclonal Antibody, Unconjugated (TMAB-02791) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 16. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AQP1 Monoclonal Antibody, Unconjugated (TMAB-02791) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 17. Paraformaldehyde-fixed, paraffin embedded Human Breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AQP1 Monoclonal Antibody, Unconjugated (TMAB-02791) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 18. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AQP1 Monoclonal Antibody, Unconjugated (TMAB-02791) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 19. 25 μg total protein per lane of various lysates (see on figure) probed with AQP1 monoclonal antibody, unconjugated (TMAB-02791) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min. 20. Paraformaldehyde-fixed, paraffin embedded Mouse Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AQP1 Monoclonal Antibody, Unconjugated (TMAB-02791) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 21. Paraformaldehyde-fixed, paraffin embedded Human Pancreatic Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AQP1 Monoclonal Antibody, Unconjugated (TMAB-02791) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. |
| Application | |
| Recommended Dose | WB: 1:1000-5000; IF: 1:200-1000; IHC-P: 1:200-1000; IHC-Fr: 1:200-1000 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Membrane; Multi-pass membrane protein. |
| Tissue Specificity | Expressed in a number of tissues including erythrocytes, renal tubules, retinal pigment epithelium, heart, lung, skeletal muscle, kidney and pancreas. Weakly expressed in brain, placenta and liver. |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | Aquaporin 1 is a 28kD integral membrane protein which was originally identified in red blood cells and renal proximal tubules. AQP1 is also expressed by the choroid plexus and various other tissues. It forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. |
| Immunogen | A synthesized peptide: human Aquaporin 1 |
| Antigen Species | Human |
| Gene Name | AQP1 |
| Gene ID | |
| Protein Name | Aquaporin-1 |
| Uniprot ID | |
| Function | Forms a water-specific channel that provides the plasmamembranes of red cells and kidney proximal tubules with highpermeability to water, thereby permitting water to move in thedirection of an osmotic gradient. |
| Molecular Weight | Theoretical: 28 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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