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Anti-APPL1 Antibody (2A728)
Catalog No. TMAB-02786 Copy Product Info
🥰Excellent
Anti-APPL1 Antibody (2A728) is a Rabbit antibody targeting APPL1. Anti-APPL1 Antibody (2A728) can be used in WB,IHC-P,IHC-Fr,IF.
Anti-APPL1 Antibody (2A728)
Copy Product Info🥰Excellent
Catalog No. TMAB-02786
Anti-APPL1 Antibody (2A728) is a Rabbit antibody targeting APPL1. Anti-APPL1 Antibody (2A728) can be used in WB,IHC-P,IHC-Fr,IF.
Anti-APPL1 Antibody
(2A728)
(2A728)
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 25 μL | $149 | 7-10 days | 7-10 days | |
| 50 μL | $262 | 7-10 days | 7-10 days | |
| 100 μL | $472 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
Stability & Storage
| Description | Anti-APPL1 Antibody (2A728) is a Rabbit antibody targeting APPL1. Anti-APPL1 Antibody (2A728) can be used in WB,IHC-P,IHC-Fr,IF. |
| Clone | 2A728 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. 25 μg total protein per lane of various lysates (see on figure) probed with APPL1 monoclonal antibody, unconjugated (TMAB-02786) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min. 2. Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with APPL1 Monoclonal Antibody, Unconjugated (TMAB-02786) at 1: 250 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Human Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with APPL1 Monoclonal Antibody, Unconjugated (TMAB-02786) at 1: 250 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with APPL1 Monoclonal Antibody, Unconjugated (TMAB-02786) at 1: 250 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with APPL1 Monoclonal Antibody, Unconjugated (TMAB-02786) at 1: 250 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with APPL1 Monoclonal Antibody, Unconjugated (TMAB-02786) at 1: 250 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Early endosome membrane; Peripheral membrane protein. Nucleus. Note=Early endosomal membrane-bound and nuclear. Translocated into the nucleus upon release from endosomal membranes following internalization of EGF. |
| Tissue Specificity | High levels in heart, ovary, pancreas and skeletal muscle. |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | The protein encoded by this gene has been shown to be involved in the regulation of cell proliferation, and in the crosstalk between the adiponectin signalling and insulin signalling pathways. The encoded protein binds many other proteins, including RAB5A, DCC, AKT2, PIK3CA, adiponectin receptors, and proteins of the NuRD/MeCP1 complex. This protein is found associated with endosomal membranes, but can be released by EGF and translocated to the nucleus. [provided by RefSeq, Jul 2008] |
| Immunogen | A synthesized peptide: human Dip13 alpha |
| Gene Name | APPL1 |
| Gene ID | |
| Protein Name | DCC-interacting protein 13-alpha |
| Function | Required for the regulation of cell proliferation in response to extracellular signals from an early endosomal compartment. Links Rab5 to nuclear signal transduction. |
| Molecular Weight | Theoretical: 80 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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