Anti-APOH Polyclonal Antibody is a Rabbit antibody targeting APOH. Anti-APOH Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat (predicted:Dog,Cow,Horse,Rabbit)

1. Tissue/cell: human laryngeal cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-APOH Polyclonal Antibody, Unconjugated (TMAB-02767) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Sample:
Lane 1: Liver (Mouse) Lysate at 40 μg
Lane 2: Liver (Rat) Lysate at 40 μg
Lane 3: Pancreas (Rat) Lysate at 40 μg
Primary: Anti-APOH (TMAB-02767) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 44-50 kD
Observed band size: 47 kD
3. Sample:
A549 Cell (Human) Lysate at 30 μg
Primary: Anti-APOH at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kD
Observed band size: 35/50 kD
4. Sample:
HepG2 Cell (Human) Lysate at 30 μg
Primary: Anti-APOH at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kD
Observed band size: 35/50 kD

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human ApoH

Human

APOH

beta-2-glycoprotein 1

Binds to various kinds of negatively charged substances such as heparin, phospholipids, and dextran sulfate. May prevent activation of the intrinsic blood coagulation cascade by binding to phospholipids on the surface of damaged cells.