Anti-APOE Antibody (3H64) is a Mouse antibody targeting APOE. Anti-APOE Antibody (3H64) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.

IgG1, Kappa

3H64

Human,Mouse

1. Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:1000
Primary ab incubation condition: room temperature 2 h
Secondary ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: Mouse serum
Protein loading quantity: 20 μg
Exposure time: 10 s
Predicted MW: 34-36 kDa
Observed MW: 34-36 kDa
2. Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:5000
Primary ab incubation condition: room temperature 2 h
Secondary ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: Human serum
Protein loading quantity: 20 μg
Exposure time: 10 s
Predicted MW: 34-36 kDa
Observed MW: 34-36 kDa
3. Cell line: HepG2
Fixative: 100% Ice-cold methanol
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary ab: Goat Anti-Mouse IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for TMAB-02765
4. Tissue: Human brain
Section type: Formalin fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit (Mouse)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for TMAB-02765
5. Tissue: Mouse brain
Section type: Formalin fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit (Mouse)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for TMAB-02765

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:50-100; IF: 1:100-500

Monoclonal

APOE

Apolipoprotein E

Mediates the binding, internalization, and catabolism of lipoprotein particles. It can serve as a ligand for the LDL (apo B/E) receptor and for the specific apo-E receptor (chylomicron remnant) of hepatic tissues.