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Anti-APOBEC3B Polyclonal Antibody
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Catalog No. TMAB-02759
Anti-APOBEC3B Polyclonal Antibody is a Rabbit antibody targeting APOBEC3B. Anti-APOBEC3B Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-APOBEC3B Polyclonal Antibody
Anti-APOBEC3B Polyclonal Antibody
Copy Product Info😃Good
Catalog No. TMAB-02759
Anti-APOBEC3B Polyclonal Antibody is a Rabbit antibody targeting APOBEC3B. Anti-APOBEC3B Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $222 | 7-10 days | 7-10 days | |
| 100 μL | $372 | 7-10 days | 7-10 days | |
| 200 μL | $528 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-APOBEC3B Polyclonal Antibody is a Rabbit antibody targeting APOBEC3B. Anti-APOBEC3B Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Rat,Mouse |
| Verified Activity | 1. Tissue/cell: rat spleen tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-APOBEC3B Polyclonal Antibody, Unconjugated (TMAB-02759) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining 2. Tissue/cell: Rat ovary tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-APOBEC3B Polyclonal Antibody, Unconjugated (TMAB-02759) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining 3. Tissue/cell: Mouse spleen tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-APOBEC3B Polyclonal Antibody, Unconjugated (TMAB-02759) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining 4. Sample: Jurkat (Human) Cell Lysate at 40 μg Primary: Anti-APOBEC3B (TMAB-02759) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 46 kD Observed band size: 46 kD |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Nucleus. |
| Tissue Specificity | Expressed at high and moderate levels in peripheral blood leukocytes, spleen, testes, heart, thymus, prostate and ovary. Also expressed at low levels in several other tissues. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | APOBEC proteins inhibit retroviruses by deaminating cytosine residues of viral RNA and DNA. The seven APOBEC3 genes or pseudogenes are found in a cluster thought to result from gene duplication on chromosome 22. APOBEC3 proteins are thought to be RNA editing enzymes and have roles in cell growth. APOBEC3B, also known as phorbolin-1-related protein or phorbolin-2/3, is a 382 amino acid protein belonging to the cytidine and deoxycytidylate deaminase family. APOBEC3B lacks cytidine deaminase activity on RNA molecules, but has been shown to bind to apoB and AU-rich RNAs. APOBEC3B forms a homodimer and interacts with APOBEC3G. APOBEC3B is expressed at at moderate levels in heart, testis, thymus, prostate, ovary, spleen and peripheral blood leukocytes. |
| Immunogen | KLH conjugated synthetic peptide: human APOBEC3B |
| Antigen Species | Human |
| Gene Name | APOBEC3B |
| Gene ID | |
| Protein Name | DNA dC->dU-editing enzyme APOBEC-3B |
| Uniprot ID | |
| Function | APOBEC3B is a member of the cytidine deaminase gene family. However, it lacks cytidine deaminase activity, at least on RNA molecules (monomeric nucleoside substrates or synthetic apoB, NF1 and NAT1 RNA template). It binds to apoB and AU-rich RNAs. It is unable to reduce HIV-1 infectivity in vitro. |
| Molecular Weight | Theoretical: 46 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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