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Anti-APOA1 Antibody (5L985) is a Rabbit antibody targeting APOA1. Anti-APOA1 Antibody (5L985) can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 25 μL | $150 | 7-10 days | 7-10 days | |
| 50 μL | $264 | 7-10 days | 7-10 days | |
| 100 μL | $470 | 7-10 days | 7-10 days |
| Description | Anti-APOA1 Antibody (5L985) is a Rabbit antibody targeting APOA1. Anti-APOA1 Antibody (5L985) can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Clone | 5L985 |
| Reactivity | Human,Mouse |
| Verified Activity | 1. Western blot analysis of HepG2 cell lysate. Using APOA1 (TMAB-02753) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min. 2. 25 μg total protein per lane of various lysates (see on figure) probed with APOA1 monoclonal antibody, unconjugated (TMAB-02753) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min. 3. Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with APOA1 Monoclonal Antibody, Unconjugated (TMAB-02753) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with APOA1 Monoclonal Antibody, Unconjugated (TMAB-02753) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; IF: 1:50-200 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Secreted. |
| Tissue Specificity | Major protein of plasma HDL, also found in chylomicrons. Synthesized in the liver and small intestine. |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 10mM phosphate buffered saline (pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| Research Background | Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility. |
| Immunogen | A synthesized peptide: human Apolipoprotein A1 |
| Antigen Species | Human |
| Gene Name | APOA1 |
| Gene ID | |
| Protein Name | Apolipoprotein A-I |
| Uniprot ID | |
| Function | Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility. |
| Molecular Weight | Theoretical: 31 kDa. Actual: 31 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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