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Anti-APLN Polyclonal Antibody

😃Good
Catalog No. TMAB-00129
Alias APJ endogenous ligand, Apelin, APEL

Anti-APLN Polyclonal Antibody is a Rabbit antibody targeting APLN. Anti-APLN Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.

Anti-APLN Polyclonal Antibody

Anti-APLN Polyclonal Antibody

😃Good
Catalog No. TMAB-00129Alias APJ endogenous ligand, Apelin, APEL
Anti-APLN Polyclonal Antibody is a Rabbit antibody targeting APLN. Anti-APLN Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$2227-10 days
100 μL$3747-10 days
200 μL$5277-10 days
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Product Introduction

Bioactivity
Description
Anti-APLN Polyclonal Antibody is a Rabbit antibody targeting APLN. Anti-APLN Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
AliasAPJ endogenous ligand, Apelin, APEL
Ig Type
IgG
Reactivity
Human,Mouse,Rat
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Apelin) Polyclonal Antibody, Unconjugated (TMAB-00129) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Apelin) Polyclonal Antibody, Unconjugated (TMAB-00129) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Application
IHC-P,IHC-Fr,IF
Recommended Dose
IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationSecreted.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundApelin (also known as APLN) is a peptide that in humans is encoded by the APLN gene. Apelin is the endogenous ligand for the G-protein-coupled APJ receptor that is expressed at the surface of some cell types. It is widely expressed in various organs such as the heart, lung, kidney, liver, adipose tissue, gastrointestinal tract, brain, adrenal glands, endothelium, and human plasma.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human Apelin
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Blood Pressure regulation,Inotropics,Obesity,More Neuropeptides,GPCR
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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