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Anti-APLN Polyclonal Antibody
😃Good
Catalog No. TMAB-00129
Alias APJ endogenous ligand, Apelin, APEL
Anti-APLN Polyclonal Antibody is a Rabbit antibody targeting APLN. Anti-APLN Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Anti-APLN Polyclonal Antibody
Anti-APLN Polyclonal Antibody
😃Good
Catalog No. TMAB-00129Alias APJ endogenous ligand, Apelin, APEL
Anti-APLN Polyclonal Antibody is a Rabbit antibody targeting APLN. Anti-APLN Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $222 | 7-10 days | |
| 100 μL | $374 | 7-10 days | |
| 200 μL | $527 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-APLN Polyclonal Antibody is a Rabbit antibody targeting APLN. Anti-APLN Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF. |
| Alias | APJ endogenous ligand, Apelin, APEL |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Apelin) Polyclonal Antibody, Unconjugated (TMAB-00129) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Apelin) Polyclonal Antibody, Unconjugated (TMAB-00129) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
| Application | |
| Recommended Dose | IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Secreted. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | Apelin (also known as APLN) is a peptide that in humans is encoded by the APLN gene. Apelin is the endogenous ligand for the G-protein-coupled APJ receptor that is expressed at the surface of some cell types. It is widely expressed in various organs such as the heart, lung, kidney, liver, adipose tissue, gastrointestinal tract, brain, adrenal glands, endothelium, and human plasma. |
| Immunogen | KLH conjugated synthetic peptide: human Apelin |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Blood Pressure regulation,Inotropics,Obesity,More Neuropeptides,GPCR |
| Molecular Weight | Theoretical: 10 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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