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Anti-APLN Polyclonal Antibody (Synonyms: APJ endogenous ligand, Apelin, APEL)

Catalog No. TMAB-00129 Copy Product Info
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Anti-APLN Polyclonal Antibody is a Rabbit antibody targeting APLN. Anti-APLN Polyclonal Antibody can be used in IF, IHC-Fr, IHC-P.

Anti-APLN Polyclonal Antibody

Copy Product Info
🥰Excellent
Catalog No. TMAB-00129
Synonyms APJ endogenous ligand, Apelin, APEL

Anti-APLN Polyclonal Antibody is a Rabbit antibody targeting APLN. Anti-APLN Polyclonal Antibody can be used in IF, IHC-Fr, IHC-P.

Anti-APLN Polyclonal Antibody
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2227-10 days7-10 days
100 μL$3747-10 days7-10 days
200 μL$5277-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-APLN Polyclonal Antibody is a Rabbit antibody targeting APLN. Anti-APLN Polyclonal Antibody can be used in IF, IHC-Fr, IHC-P.
SynonymsAPJ endogenous ligand, Apelin, APEL
Ig Type
IgG
Reactivity
Mouse,Rat (predicted:Human)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Apelin) Polyclonal Antibody, Unconjugated (TMAB-00129) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Apelin) Polyclonal Antibody, Unconjugated (TMAB-00129) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
verifiedActivityverifiedActivity
Application
Recommended Dose
IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationSecreted.
Tissue SpecificityExpressed in the brain with highest levels in the frontal cortex, thalamus, hypothalamus and midbrain. Secreted by the mammary gland into the colostrum and the milk.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1 mg/mL
Research BackgroundApelin (also known as APLN) is a peptide that in humans is encoded by the APLN gene. Apelin is the endogenous ligand for the G-protein-coupled APJ receptor that is expressed at the surface of some cell types. It is widely expressed in various organs such as the heart, lung, kidney, liver, adipose tissue, gastrointestinal tract, brain, adrenal glands, endothelium, and human plasma.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human Apelin
Antigen Species
Human
Gene Name
APLN
Gene ID
Protein Name
Apelin
Uniprot ID
Biology Area
Blood Pressure regulation,Inotropics,Obesity,More Neuropeptides,GPCR
Function
Endogenous ligand for APJ, an alternative coreceptor with CD4 for HIV-1 infection. Inhibits HIV-1 entry in cells coexpressing CD4 and APJ. Apelin-36 has a greater inhibitory activity on HIV infection than other synthetic apelin derivatives. The oral intake in the colostrum and the milk could have a role in the modulation of the immune responses in neonates. May also have a role in the central control of body fluid homeostasis by influencing AVP release and drinking behavior.
Chemical Properties
Molecular WeightTheoretical: 10 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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