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Anti-Apg7 Antibody (7C583)

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Catalog No. TMAC-00243
Alias Ubiquitin activating enzyme E1 like protein, hAGP7, GSA7, GSA 7, DKFZp434N0735, Autophagy-related 7(yeast), Autophagy related protein 7, Autophagy 7, S. cerevisiae, homolog of, Atg7l, ATG7 autophagy related 7 homolog(S. cerevisiae), ATG7 autophagy related 7 homolog, ATG 7, APG7L, APG7, S. cerevisiae, homolog of, APG7 like, APG7 autophagy 7-like(S. cerevisiae), APG7 autophagy 7 like, Apg 7

Anti-Apg7 Antibody (7C583) is a Rabbit antibody targeting Apg7. Anti-Apg7 Antibody (7C583) can be used in FCM,ICC/IF,IHC,IP,WB.

Anti-Apg7 Antibody (7C583)

Anti-Apg7 Antibody (7C583)

😃Good
Catalog No. TMAC-00243Alias Ubiquitin activating enzyme E1 like protein, hAGP7, GSA7, GSA 7, DKFZp434N0735, Autophagy-related 7(yeast), Autophagy related protein 7, Autophagy 7, S. cerevisiae, homolog of, Atg7l, ATG7 autophagy related 7 homolog(S. cerevisiae), ATG7 autophagy related 7 homolog, ATG 7, APG7L, APG7, S. cerevisiae, homolog of, APG7 like, APG7 autophagy 7-like(S. cerevisiae), APG7 autophagy 7 like, Apg 7
Anti-Apg7 Antibody (7C583) is a Rabbit antibody targeting Apg7. Anti-Apg7 Antibody (7C583) can be used in FCM,ICC/IF,IHC,IP,WB.
Pack SizePriceAvailabilityQuantity
50 μL$2987-10 days
100 μL$4977-10 days
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Product Introduction

Bioactivity
Description
Anti-Apg7 Antibody (7C583) is a Rabbit antibody targeting Apg7. Anti-Apg7 Antibody (7C583) can be used in FCM,ICC/IF,IHC,IP,WB.
SynonymsUbiquitin activating enzyme E1 like protein, hAGP7, GSA7, GSA 7, DKFZp434N0735, Autophagy-related 7(yeast), Autophagy related protein 7, Autophagy 7, S. cerevisiae, homolog of, Atg7l, ATG7 autophagy related 7 homolog(S. cerevisiae), ATG7 autophagy related 7 homolog, ATG 7, APG7L, APG7, S. cerevisiae, homolog of, APG7 like, APG7 autophagy 7-like(S. cerevisiae), APG7 autophagy 7 like, Apg 7
Ig Type
IgG
Clone
7C583
Reactivity
Human
Verified Activity
1. Western blot analysis of Apg7 on Jurkat cells lysates using anti-Apg7 antibody at 1/1,000 dilution.
2. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Apg7 antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Apg7 antibody. Counter stained with hematoxylin.
4. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Apg7 antibody. Counter stained with hematoxylin.
5. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Apg7 antibody. Counter stained with hematoxylin.
6. ICC staining Apg7 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
7. ICC staining Apg7 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
8. Flow cytometric analysis of Hela cells with Apg7 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Application
Recommended Dose
WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionRecombinant Antibody
PurificationProA affinity purified
AppearanceLiquid
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Research BackgroundIn yeast, autophagy is an essential process for survival during nutrient starvation and cell differentiation. The process of autophagy is characterized as a non-selective degradation of cytoplasmic proteins into membrane stuctures called autophagosomes, and it is dependent on several proteins, including the autophagy proteins APG5 and APG7. Yeast Apg7 and the human homolog, APG7, share similarities with the ubiquitin-activating enzyme E1 in Saccharomyces cerevisiae, and are likewise responsible for enzymatically activating the autophagy conjugation system. Apg5 and the human homolog, APG5 (also designated apoptosis specific protein or APS), function as substrates for the autophagy protein APG12. These proteins are covalently bonded together to form APG12/APG5 conjugates, which are required for the progression of autophagy.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 78 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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